2MK7
Tetra-O-GalNAc glycosylated mucin sequence from alpha dystroglycan mucin domain
Summary for 2MK7
| Entry DOI | 10.2210/pdb2mk7/pdb |
| NMR Information | BMRB: 19759 |
| Descriptor | Alpha-dystroglycan, 2-acetamido-2-deoxy-alpha-D-galactopyranose (2 entities in total) |
| Functional Keywords | mucin domain, glycoprotein, glycosylation, alpha-dystroglycan, tn antigen, n-acetyl-galactosamine, structural protein |
| Biological source | Homo sapiens (human) |
| Total number of polymer chains | 1 |
| Total formula weight | 1881.01 |
| Authors | Live, D.,Borgert, A. (deposition date: 2014-02-02, release date: 2015-02-04, Last modification date: 2024-11-06) |
| Primary citation | Borgert, A.,Foley, B.L.,Live, D. Contrasting the conformational effects of alpha-O-GalNAc and alpha-O-Man glycan protein modifications and their impact on the mucin-like region of alpha-dystroglycan. Glycobiology, 31:649-661, 2021 Cited by PubMed Abstract: We have carried out a comparative study of the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan. Both such modifications can coexist in this domain of the glycoprotein. Solution NMR experiments and molecular dynamics calculations were employed. Comparing the results for an unmodified peptide Ac- PPTTTTKKP-NH2 sequence from α-dystroglycan, and glycoconjugates with either modification on the Ts, we find that the impact of the α-O-Man modification on the peptide scaffold is quite limited, while that of the α-O-GalNAc is more profound. The results for the α-O-GalNAc glycoconjugate are consistent with what has been seen earlier in other systems. Further examination of the NMR-based structure and the MD results suggest a more extensive network of hydrogen bond interactions within the α-O-GalNAc-threonine residue than has been previously appreciated, which influences the properties of the protein backbone. The conformational effects are relevant to the mechanical properties of α-dystroglycan. PubMed: 33295623DOI: 10.1093/glycob/cwaa112 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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