2MK5
Solution structure of a protein domain
2MK5 の概要
エントリーDOI | 10.2210/pdb2mk5/pdb |
関連するPDBエントリー | 4OLK 4OLS |
NMR情報 | BMRB: 19752 |
分子名称 | Endolysin (1 entity in total) |
機能のキーワード | hydrolase |
由来する生物種 | Staphylococcus phage GH15 |
タンパク質・核酸の鎖数 | 1 |
化学式量合計 | 14048.75 |
構造登録者 | |
主引用文献 | Gu, J.,Feng, Y.,Feng, X.,Sun, C.,Lei, L.,Ding, W.,Niu, F.,Jiao, L.,Yang, M.,Li, Y.,Liu, X.,Song, J.,Cui, Z.,Han, D.,Du, C.,Yang, Y.,Ouyang, S.,Liu, Z.J.,Han, W. Structural and biochemical characterization reveals LysGH15 as an unprecedented "EF-hand-like" calcium-binding phage lysin. Plos Pathog., 10:e1004109-e1004109, 2014 Cited by PubMed Abstract: The lysin LysGH15, which is derived from the staphylococcal phage GH15, demonstrates a wide lytic spectrum and strong lytic activity against methicillin-resistant Staphylococcus aureus (MRSA). Here, we find that the lytic activity of the full-length LysGH15 and its CHAP domain is dependent on calcium ions. To elucidate the molecular mechanism, the structures of three individual domains of LysGH15 were determined. Unexpectedly, the crystal structure of the LysGH15 CHAP domain reveals an "EF-hand-like" calcium-binding site near the Cys-His-Glu-Asn quartet active site groove. To date, the calcium-binding site in the LysGH15 CHAP domain is unique among homologous proteins, and it represents the first reported calcium-binding site in the CHAP family. More importantly, the calcium ion plays an important role as a switch that modulates the CHAP domain between the active and inactive states. Structure-guided mutagenesis of the amidase-2 domain reveals that both the zinc ion and E282 are required in catalysis and enable us to propose a catalytic mechanism. Nuclear magnetic resonance (NMR) spectroscopy and titration-guided mutagenesis identify residues (e.g., N404, Y406, G407, and T408) in the SH3b domain that are involved in the interactions with the substrate. To the best of our knowledge, our results constitute the first structural information on the biochemical features of a staphylococcal phage lysin and represent a pivotal step forward in understanding this type of lysin. PubMed: 24831957DOI: 10.1371/journal.ppat.1004109 主引用文献が同じPDBエントリー |
実験手法 | SOLUTION NMR |
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