2MJX

Solution NMR structure of a mismatch DNA

Summary for 2MJX

• Entry DOI: 10.2210/pdb2mjx/pdb
• NMR Information: BMRB: 19745
• Descriptor: DNA (5'-D(*GP*CP*GP*CP*AP*TP*GP*CP*TP*AP*CP*GP*CP*G)-3'), DNA (5'-D(*CP*GP*CP*GP*TP*AP*CP*GP*AP*TP*GP*CP*GP*C)-3') (2 entities in total)
• Functional Keywords: mismatch dna, g-g mismatch, dna
• Biological source: synthetic; More
• Total number of polymer chains: 2
• Total formula weight: 8563.56

Authors

Ghosh, A.,Kumar, K.R.,Bhunia, A.,Chatterjee, S. (deposition date: 2014-01-21, release date: 2014-03-05, Last modification date: 2024-05-15)

Primary citation

Ghosh, A.,Kar, R.K.,Krishnamoorthy, J.,Chatterjee, S.,Bhunia, A.
Double GC:GC mismatch in dsDNA enhances local dynamics retaining the DNA footprint: a high-resolution NMR study
Chemmedchem, 9:2059-2064, 2014

PubMed Abstract: Mutations in the genome are responsible for several fatal genetic disorders. The default DNA repair mechanism restores the malfunction of the gene caused by mutation to maintain functional regularity and sequential integrity of the cell. Here, we have elucidated the NMR structure and the dynamics of GC mismatched DNA (PDB code: 2MJX) and found that the mismatched DNA still retains the typical B-type helical form, but in the process introduces backbone distortion resulting from frame-shifted base pairs.

PubMed: 25080019
DOI: 10.1002/cmdc.201402238

Experimental method

SOLUTION NMR