2MJX

Solution NMR structure of a mismatch DNA

2MJX の概要

• エントリーDOI: 10.2210/pdb2mjx/pdb
• NMR情報: BMRB: 19745
• 分子名称: DNA (5'-D(*GP*CP*GP*CP*AP*TP*GP*CP*TP*AP*CP*GP*CP*G)-3'), DNA (5'-D(*CP*GP*CP*GP*TP*AP*CP*GP*AP*TP*GP*CP*GP*C)-3') (2 entities in total)
• 機能のキーワード: mismatch dna, g-g mismatch, dna
• 由来する生物種: synthetic; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 8563.56

構造登録者

Ghosh, A.,Kumar, K.R.,Bhunia, A.,Chatterjee, S. (登録日: 2014-01-21, 公開日: 2014-03-05, 最終更新日: 2024-05-15)

主引用文献

Ghosh, A.,Kar, R.K.,Krishnamoorthy, J.,Chatterjee, S.,Bhunia, A.
Double GC:GC mismatch in dsDNA enhances local dynamics retaining the DNA footprint: a high-resolution NMR study
Chemmedchem, 9:2059-2064, 2014

PubMed Abstract: Mutations in the genome are responsible for several fatal genetic disorders. The default DNA repair mechanism restores the malfunction of the gene caused by mutation to maintain functional regularity and sequential integrity of the cell. Here, we have elucidated the NMR structure and the dynamics of GC mismatched DNA (PDB code: 2MJX) and found that the mismatched DNA still retains the typical B-type helical form, but in the process introduces backbone distortion resulting from frame-shifted base pairs.

PubMed: 25080019
DOI: 10.1002/cmdc.201402238

実験手法

SOLUTION NMR