2MJS

Anoplin R5K T8W in DPC micelles

2MJS の概要

• エントリーDOI: 10.2210/pdb2mjs/pdb
• 関連するPDBエントリー: 2MJQ 2MJR 2MJT
• NMR情報: BMRB: 11553
• 分子名称: Anoplin, UNKNOWN ATOM OR ION, dodecyl 2-(trimethylammonio)ethyl phosphate (3 entities in total)
• 機能のキーワード: amphipathic helix, antimicrobial protein
• 由来する生物種: Anoplius samariensis (Solitary wasp)
• 細胞内の位置: Secreted: P0C005
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24057.64

構造登録者

Uggerhoej, L.,Poulsen, T.J.,Wimmer, R. (登録日: 2014-01-16, 公開日: 2014-12-03, 最終更新日: 2024-11-20)

主引用文献

Uggerhj, L.E.,Poulsen, T.J.,Munk, J.K.,Fredborg, M.,Sondergaard, T.E.,Frimodt-Moller, N.,Hansen, P.R.,Wimmer, R.
Rational Design of Alpha-Helical Antimicrobial Peptides: Do's and Don'ts.
Chembiochem, 16:242-253, 2015

PubMed Abstract: Antimicrobial peptides (AMPs) are promising candidates for battling multiresistant bacteria. Despite extensive research, structure-activity relationships of AMPs are not fully understood, and there is a lack of structural data relating to AMPs in lipids. Here we present the NMR structure of anoplin (GLLKRIKTLL-NH2 ) in a micellar environment. A vast library of substitutions was designed and tested for antimicrobial and hemolytic activity, as well as for changes in structure and lipid interactions. This showed that improvement of antimicrobial activity without concomitant introduction of strong hemolytic activity can be achieved through subtle increases in the hydrophobicity of the hydrophobic face or through subtle increases in the polarity of the hydrophilic face of the helix, or-most efficiently-a combination of both. A set of guidelines based on the results is given, for assistance in how to modify cationic α-helical AMPs in order to control activity and selectivity. The guidelines are finally tested on a different peptide.

PubMed: 25530580
DOI: 10.1002/cbic.201402581

実験手法

SOLUTION NMR