2MIM

NMR structure of the chicken CD3 epsilon delta/gamma heterodimer

2MIM の概要

• エントリーDOI: 10.2210/pdb2mim/pdb
• NMR情報: BMRB: 19687
• 分子名称: CD3 epsilon protein,CD3 glycoprotein (1 entity in total)
• 機能のキーワード: immune signalling subunit, cd3, tcr, immune system
• 由来する生物種: Gallus gallus (Chicken); 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19569.77

構造登録者

Headey, S.,Berry, R.,Rossjohn, J. (登録日: 2013-12-15, 公開日: 2014-02-12, 最終更新日: 2024-11-20)

主引用文献

Berry, R.,Headey, S.J.,Call, M.J.,McCluskey, J.,Tregaskes, C.A.,Kaufman, J.,Koh, R.,Scanlon, M.J.,Call, M.E.,Rossjohn, J.
Structure of the chicken CD3 epsilon delta / gamma heterodimer and its assembly with the alpha beta T cell receptor
J.Biol.Chem., 289:8240-8251, 2014

PubMed Abstract: In mammals, the αβT cell receptor (TCR) signaling complex is composed of a TCRαβ heterodimer that is noncovalently coupled to three dimeric signaling molecules, CD3εδ, CD3εγ, and CD3ζζ. The nature of the TCR signaling complex and subunit arrangement in different species remains unclear however. Here we present a structural and biochemical analysis of the more primitive ancestral form of the TCR signaling complex found in chickens. In contrast to mammals, chickens do not express separate CD3δ and CD3γ chains but instead encode a single hybrid chain, termed CD3δ/γ, that is capable of pairing with CD3ε. The NMR structure of the chicken CD3εδ/γ heterodimer revealed a unique dimer interface that results in a heterodimer with considerable deviation from the distinct side-by-side architecture found in human and murine CD3εδ and CD3εγ. The chicken CD3εδ/γ heterodimer also contains a unique molecular surface, with the vast majority of surface-exposed, nonconserved residues being clustered to a single face of the heterodimer. Using an in vitro biochemical assay, we demonstrate that CD3εδ/γ can assemble with both chicken TCRα and TCRβ via conserved polar transmembrane sites. Moreover, analogous to the human TCR signaling complex, the presence of two copies of CD3εδ/γ is required for ζζ assembly. These data provide insight into the evolution of this critical receptor signaling apparatus.

PubMed: 24488493
DOI: 10.1074/jbc.M113.544965

実験手法

SOLUTION NMR