2MH2

Structural insights into the DNA recognition and protein interaction domains reveal fundamental homologous DNA pairing properties of HOP2

2MH2 の概要

• エントリーDOI: 10.2210/pdb2mh2/pdb
• NMR情報: BMRB: 19613
• 分子名称: Homologous-pairing protein 2 homolog (1 entity in total)
• 機能のキーワード: meiosis, homologous recombination, homologous pairing protein, dna binding protein
• 由来する生物種: Mus musculus (mouse)
• 細胞内の位置: Nucleus: O35047
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9257.39

構造登録者

Moktan, H.,Guiraldelli, M.F.,Eyter, C.A.,Zhao, W.,Camerini-Otero, R.D.,Sung, P.,Zhou, D.H.,Pezza, R.J. (登録日: 2013-11-13, 公開日: 2014-04-16, 最終更新日: 2024-05-01)

主引用文献

Moktan, H.,Guiraldelli, M.F.,Eyster, C.A.,Zhao, W.,Lee, C.Y.,Mather, T.,Camerini-Otero, R.D.,Sung, P.,Zhou, D.H.,Pezza, R.J.
Solution Structure and DNA-binding Properties of the Winged Helix Domain of the Meiotic Recombination HOP2 Protein.
J.Biol.Chem., 289:14682-14691, 2014

PubMed Abstract: The HOP2 protein is required for efficient double-strand break repair which ensures the proper synapsis of homologous chromosomes and normal meiotic progression. We previously showed that in vitro HOP2 shows two distinctive activities: when it is incorporated into a HOP2-MND1 heterodimer, it stimulates DMC1 and RAD51 recombination activities, and the purified HOP2 alone is proficient in promoting strand invasion. The structural and biochemical basis of HOP2 action in recombination are poorly understood; therefore, they are the focus of this work. Herein, we present the solution structure of the amino-terminal portion of mouse HOP2, which contains a typical winged helix DNA-binding domain. Together with NMR spectral changes in the presence of double-stranded DNA, protein docking on DNA, and mutation analysis to identify the amino acids involved in DNA coordination, our results on the three-dimensional structure of HOP2 provide key information on the fundamental structural and biochemical requirements directing the interaction of HOP2 with DNA. These results, in combination with mutational experiments showing the role of a coiled-coil structural feature involved in HOP2 self-association, allow us to explain important aspects of the function of HOP2 in recombination.

PubMed: 24711446
DOI: 10.1074/jbc.M114.548180

実験手法

SOLUTION NMR