2MGX

NMR structure of SRA1p C-terminal domain

2MGX の概要

• エントリーDOI: 10.2210/pdb2mgx/pdb
• NMR情報: BMRB: 19607
• 分子名称: Steroid receptor RNA activator 1 (1 entity in total)
• 機能のキーワード: unknown function
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: Q9HD15
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14769.75

構造登録者

Bilinovich, S.M.,Davis, C.M.,Morris, D.L.,Ray, L.A.,Prokop, J.W.,Buchan, G.J.,Leeper, T.C. (登録日: 2013-11-10, 公開日: 2014-02-12, 最終更新日: 2024-05-15)

主引用文献

Bilinovich, S.M.,Davis, C.M.,Morris, D.L.,Ray, L.A.,Prokop, J.W.,Buchan, G.J.,Leeper, T.C.
The C-Terminal Domain of SRA1p Has a Fold More Similar to PRP18 than to an RRM and Does Not Directly Bind to the SRA1 RNA STR7 Region.
J.Mol.Biol., 426:1753-1765, 2014

PubMed Abstract: Steroid receptor activator RNA protein (SRA1p) is the translation product of the bi-functional long non-coding RNA steroid receptor activator RNA 1 (SRA1) that is part of the steroid receptor coactivator-1 acetyltransferase complex and is indicated to be an epigenetic regulatory component. Previously, the SRA1p protein was suggested to contain an RNA recognition motif (RRM) domain. We have determined the solution structure of the C-terminal domain of human SRA1p by NMR spectroscopy. Our structure along with sequence comparisons among SRA1p orthologs and against authentic RRM proteins indicates that it is not an RRM domain but rather an all-helical protein with a fold more similar to the PRP18 splicing factor. NMR spectroscopy on the full SRA1p protein suggests that this structure is relevant to the native full-length context. Furthermore, molecular modeling indicates that this fold is well conserved among vertebrates. Amino acid variations in this protein seen across sequenced human genomes, including those in tumor cells, indicate that mutations that disrupt the fold occur vary rarely and highlight that its function is well conserved. SRA1p had previously been suggested to bind to the SRA1 RNA, but NMR spectra of SRA1p in the presence of its 80-nt RNA target suggest otherwise and indicate that this protein must be part of a multi-protein complex in order to recognize its proposed RNA recognition element.

PubMed: 24486611
DOI: 10.1016/j.jmb.2014.01.007

実験手法

SOLUTION NMR