2MGR

Structure of Plasmodium Yoelii Merozoite Surface Protein 1 - C-terminal Domain, E28K mutant

2MGR の概要

• エントリーDOI: 10.2210/pdb2mgr/pdb
• 関連するPDBエントリー: 2MGP
• NMR情報: BMRB: 19234
• 分子名称: Merozoite surface protein 1 (1 entity in total)
• 機能のキーワード: membrane protein
• 由来する生物種: Plasmodium yoelii yoelii
• 細胞内の位置: Cell membrane; Lipid-anchor, GPI-anchor (By similarity): P13828
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10729.79

構造登録者

Curd, R.D.,Birdsall, B.,Kadekoppala, M.,Ogun, S.,Kelly, G.,Holder, A.A. (登録日: 2013-11-04, 公開日: 2014-02-12, 最終更新日: 2024-05-15)

主引用文献

Curd, R.D.,Birdsall, B.,Kadekoppala, M.,Ogun, S.A.,Kelly, G.,Holder, A.A.
The structure of Plasmodium yoelii merozoite surface protein 119, antibody specificity and implications for malaria vaccine design
OPEN BIOLOGY, 4:130091-130091, 2014

PubMed Abstract: Merozoite surface protein 1 (MSP1) has been identified as a target antigen for protective immune responses against asexual blood stage malaria, but effective vaccines based on MSP1 have not been developed so far. We have modified the sequence of Plasmodium yoelii MSP119 (the C-terminal region of the molecule) and examined the ability of the variant proteins to bind protective monoclonal antibodies and to induce protection by immunization. In parallel, we examined the structure of the protein and the consequences of the amino acid changes. Naturally occurring sequence polymorphisms reduced the binding of individual protective antibodies, indicating that they contribute to immune evasion, but immunization with these variant proteins still provided protective immunity. One variant that resulted in the localized distortion of a loop close to the N-terminus of MSP119 almost completely ablated protection by immunization, indicating the importance of this region of MSP119 as a target for protective immunity and in vaccine development.

PubMed: 24403012
DOI: 10.1098/rsob.130091

実験手法

SOLUTION NMR