2MFP

Solution structure of the circular g-domain analog from the wheat metallothionein Ec-1

2MFP の概要

• エントリーDOI: 10.2210/pdb2mfp/pdb
• 関連するPDBエントリー: 2L61 2L62
• NMR情報: BMRB: 19557
• 分子名称: EC protein I/II, CADMIUM ION (2 entities in total)
• 機能のキーワード: metallothionein, metal-thiolate cluster, backbone cyclized protein, metal binding protein
• 由来する生物種: Triticum aestivum (Canadian hard winter wheat,common wheat,wheat)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3016.96

構造登録者

Tarasava, K.,Johannsen, S.,Freisinger, E. (登録日: 2013-10-14, 公開日: 2013-11-27, 最終更新日: 2024-11-06)

主引用文献

Tarasava, K.,Johannsen, S.,Freisinger, E.
Solution Structure of the Circular gamma-Domain Analog from the Wheat Metallothionein Ec-1.
Molecules, 18:14414-14429, 2013

PubMed Abstract: The first cyclic analog of a metallothionein (MT) was prepared and analyzed by UV and (magnetic) circular dichroism spectroscopy, ESI-MS as well as NMR spectroscopy. Results reveal that the evaluated cyclic γ-E(c)-1 domain of the wheat MT E(c)-1 retains its ability to coordinate two Zn(II) or Cd(II) ions and adopts a three-dimensional structure that is highly similar to the one of the linear wild-type form. However, the reduced flexibility of the protein backbone facilitates structure solution significantly and results in a certain stabilization of metal binding to the protein.

PubMed: 24284492
DOI: 10.3390/molecules181114414

実験手法

SOLUTION NMR