2MFL

Domain 2 of E. coli ribosomal protein S1

2MFL の概要

• エントリーDOI: 10.2210/pdb2mfl/pdb
• 関連するPDBエントリー: 2KHI 2KHJ
• NMR情報: BMRB: 19554
• 分子名称: 30S ribosomal protein S1 (1 entity in total)
• 機能のキーワード: s1, ribosome binding, translation initiation, rna binding, ribosomal protein
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19983.51

構造登録者

Giraud, P.,Crechet, J.,Bontems, F.,Uzan, M.,Sizun, C. (登録日: 2013-10-12, 公開日: 2014-05-21, 最終更新日: 2024-05-15)

主引用文献

Giraud, P.,Crechet, J.B.,Uzan, M.,Bontems, F.,Sizun, C.
Resonance assignment of the ribosome binding domain of E. coli ribosomal protein S1.
Biomol.Nmr Assign., 9:107-111, 2015

PubMed Abstract: Ribosomal protein S1 is an essential actor for protein synthesis in Escherichia coli. It is involved in mRNA recruitment by the 30S ribosomal subunit and recognition of the correct start codon during translation initiation. E. coli S1 is a modular protein that contains six repeats of an S1 motif, which have distinct functions despite structural homology. Whereas the three central repeats have been shown to be involved in mRNA recognition, the two first repeats that constitute the N-terminal domain of S1 are responsible for binding to the 30S subunit. Here we report the almost complete (1)H, (13)C and (15)N resonance assignment of two fragments of the 30S binding region of S1. The first fragment comprises only the first repeat. The second corresponds to the entire ribosome binding domain. Since S1 is absent from all high resolution X-ray structures of prokaryotic ribosomes, these data provide a first step towards atomic level structural characterization of this domain by NMR. Chemical shift analysis of the first repeat provides evidence for structural divergence from the canonical OB-fold of an S1 motif. In contrast the second domain displays the expected topology for an S1 motif, which rationalizes the functional specialization of the two subdomains.

PubMed: 24682851
DOI: 10.1007/s12104-014-9554-2

実験手法

SOLUTION NMR