2MFH

Csr/Rsm protein-RNA recognition - A molecular affinity ruler: RsmZ(36-44)/RsmE(dimer) 2:1 complex

2MFH の概要

• エントリーDOI: 10.2210/pdb2mfh/pdb
• 関連するPDBエントリー: 2MFC 2MFE 2MFF 2MFG
• NMR情報: BMRB: 19549
• 分子名称: Carbon storage regulator homolog, RsmZ(36-44) RNA (2 entities in total)
• 機能のキーワード: csra, rsma, rsme, rsmz, csrb, translation repressor protein, translation activation, protein sequestration, bacterial protein, non-coding rna, srna, pseudomonas aeruginosa, rna-binding proteins, messenger rna, modulation of binding affinity, molecular mimicry, translation-rna complex, translation/rna
• 由来する生物種: Pseudomonas fluorescens
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 21407.44

構造登録者

Duss, O.,Diarra Dit Konte, N.,Michel, E.,Schubert, M.,Allain, F.H.-T. (登録日: 2013-10-11, 公開日: 2014-02-26, 最終更新日: 2024-05-01)

主引用文献

Duss, O.,Michel, E.,Diarra Dit Konte, N.,Schubert, M.,Allain, F.H.
Molecular basis for the wide range of affinity found in Csr/Rsm protein-RNA recognition.
Nucleic Acids Res., 42:5332-5346, 2014

PubMed Abstract: The carbon storage regulator/regulator of secondary metabolism (Csr/Rsm) type of small non-coding RNAs (sRNAs) is widespread throughout bacteria and acts by sequestering the global translation repressor protein CsrA/RsmE from the ribosome binding site of a subset of mRNAs. Although we have previously described the molecular basis of a high affinity RNA target bound to RsmE, it remains unknown how other lower affinity targets are recognized by the same protein. Here, we have determined the nuclear magnetic resonance solution structures of five separate GGA binding motifs of the sRNA RsmZ of Pseudomonas fluorescens in complex with RsmE. The structures explain how the variation of sequence and structural context of the GGA binding motifs modulate the binding affinity for RsmE by five orders of magnitude (∼10 nM to ∼3 mM, Kd). Furthermore, we see that conformational adaptation of protein side-chains and RNA enable recognition of different RNA sequences by the same protein contributing to binding affinity without conferring specificity. Overall, our findings illustrate how the variability in the Csr/Rsm protein-RNA recognition allows a fine-tuning of the competition between mRNAs and sRNAs for the CsrA/RsmE protein.

PubMed: 24561806
DOI: 10.1093/nar/gku141

実験手法

SOLUTION NMR