2MF2

Structural and biophysical characterization of the mRNA interferase SaMazF from Staphylococcus aureus.

2MF2 の概要

• エントリーDOI: 10.2210/pdb2mf2/pdb
• 関連するPDBエントリー: 4MZM 4MZP 4MZT
• NMR情報: BMRB: 17288
• 分子名称: mRNA interferase MazF (1 entity in total)
• 機能のキーワード: ccdb/mazf fold, ribonuclease, hydrolase
• 由来する生物種: Staphylococcus aureus (Staphylococcus aureus)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 29642.02

構造登録者

Zorzini, V.,Cheung, A.,Loris, R.,van Nuland, N.A.J. (登録日: 2013-10-03, 公開日: 2014-05-21, 最終更新日: 2024-05-15)

主引用文献

Zorzini, V.,Buts, L.,Sleutel, M.,Garcia-Pino, A.,Talavera, A.,Haesaerts, S.,De Greve, H.,Cheung, A.,van Nuland, N.A.J.,Loris, R.
Structural and biophysical characterization of Staphylococcus aureus SaMazF shows conservation of functional dynamics
Nucleic Acids Res., 5:157-160, 2014

PubMed Abstract: MazF proteins are ribonucleases that cleave mRNA with high sequence-specificity as part of bacterial stress response and that are neutralized by the action of the corresponding antitoxin MazE. Prolonged activation of the toxin MazF leads to cell death. Several mazEF modules from gram-negative bacteria have been characterized in terms of catalytic activity, auto-regulation mechanism and structure, but less is known about their distant relatives found in gram-positive organisms. Currently, no solution NMR structure is available for any wild-type MazF toxin. Here we report the (1)H, (15)N and (13)C backbone and side-chain chemical shift assignments of this toxin from the pathogen bacterium Staphylococcus aureus. The BMRB accession number is 17288.

PubMed: 21213075
DOI: 10.1007/s12104-010-9290-1

実験手法

SOLUTION NMR