2MEX

Structure of the tetrameric building block of the Salmonella Typhimurium PrgI Type three secretion system needle

2MEX の概要

• エントリーDOI: 10.2210/pdb2mex/pdb
• 関連するPDBエントリー: 2lpz
• NMR情報: BMRB: 18276
• 分子名称: Protein PrgI (1 entity in total)
• 機能のキーワード: secretion, needle, filament, protein transport
• 由来する生物種: Salmonella enterica subsp. enterica serovar Typhimurium
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 35459.47

構造登録者

Loquet, A.,Habenstein, B.,Chevelkov, V.,Giller, K.,Becker, S.,Lange, A. (登録日: 2013-10-01, 公開日: 2013-12-25, 最終更新日: 2024-05-01)

主引用文献

Loquet, A.,Habenstein, B.,Chevelkov, V.,Vasa, S.K.,Giller, K.,Becker, S.,Lange, A.
Atomic structure and handedness of the building block of a biological assembly.
J.Am.Chem.Soc., 135:19135-19138, 2013

PubMed Abstract: Noncovalent supramolecular assemblies possess in general several unique subunit-subunit interfaces.The basic building block of such an assembly consists of several subunits and contains all unique interfaces. Atomic-resolution structures of monomeric subunits are typically accessed by crystallography or solution NMR and fitted into electron microscopy density maps. However, the structure of the intact building block in the assembled state remains unknown with this hybrid approach. Here, we present the solid-state NMR atomic structure of the building block of the type III secretion system needle. The building block structure consists of a homotetrameric subunit complex with three unique supramolecular interfaces. Side-chain positions at the interfaces were solved at atomic detail. The high-resolution structure reveals unambiguously the helical handedness of the assembly, determined to be right-handed for the type III secretion system needle.Additionally, the axial rise per subunit could be extracted from the tetramer structure and independently validated by mass-per-length measurements.

PubMed: 24328247
DOI: 10.1021/ja411362q

実験手法

SOLID-STATE NMR