2MEB

CHANGES IN CONFORMATIONAL STABILITY OF A SERIES OF MUTANT HUMAN LYSOZYMES AT CONSTANT POSITIONS

2MEB の概要

• エントリーDOI: 10.2210/pdb2meb/pdb
• 分子名称: LYSOZYME, SODIUM ION (3 entities in total)
• 機能のキーワード: enzyme, hydrolase, o-glycosyl, alpha + beta, glycosidase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted: P61626
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14743.68

構造登録者

Funahashi, J.,Takano, K.,Yamagata, Y.,Yutani, K. (登録日: 1998-05-02, 公開日: 1998-07-15, 最終更新日: 2024-10-30)

主引用文献

Funahashi, J.,Takano, K.,Yamagata, Y.,Yutani, K.
Contribution of amino acid substitutions at two different interior positions to the conformational stability of human lysozyme
Protein Eng., 12:841-850, 1999

PubMed Abstract: To elucidate correlative relationships between structural change and thermodynamic stability in proteins, a series of mutant human lysozymes modified at two buried positions (Ile56 and Ile59) were examined. Their thermodynamic parameters of denaturation and crystal structures were studied by calorimetry and X-ray crystallography. The mutants at positions 56 and 59 exhibited different responses to a series of amino acid substitutions. The changes in stability due to substitutions showed a linear correlation with changes in hydrophobicity of substituted residues, having different slopes at each mutation site. However, the stability of each mutant was found to be represented by a unique equation involving physical properties calculated from mutant structures. By fitting present and previous stability data for mutant human lysozymes substituted at various positions to the equation, the magnitudes of the hydrophobicity of a carbon atom and the hydrophobicity of nitrogen and neutral oxygen atoms were found to be 0.178 and -0.013 kJ/mol.A(2), respectively. It was also found that the contribution of a hydrogen bond with a length of 3.0 A to protein stability was 5.1 kJ/mol and the entropy loss of newly introduction of a water molecules was 7.8 kJ/mol.

PubMed: 10556244
DOI: 10.1093/protein/12.10.841

実験手法

X-RAY DIFFRACTION (1.8 Å)