2MDU

Circular Permutant of the WW Domain with Loop 1 Excised

2MDU の概要

• エントリーDOI: 10.2210/pdb2mdu/pdb
• 関連するPDBエントリー: 1pin 2kcf
• NMR情報: BMRB: 19503
• 分子名称: Pin1 WW domain (1 entity in total)
• 機能のキーワード: circular permutation, miniprotein, dynamics, folding, isomerase
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3794.39

構造登録者

Kier, B.L. (登録日: 2013-09-18, 公開日: 2014-01-15, 最終更新日: 2024-05-01)

主引用文献

Kier, B.L.,Anderson, J.M.,Andersen, N.H.
Circular Permutation of a WW Domain: Folding Still Occurs after Excising the Turn of the Folding-Nucleating Hairpin.
J.Am.Chem.Soc., 136:741-749, 2014

PubMed Abstract: A hyperstable Pin1 WW domain has been circularly permuted via excision of the fold-nucleating turn; it still folds to form the native three-strand sheet and hydrophobic core features. Multiprobe folding dynamics studies of the normal and circularly permuted sequences, as well as their constituent hairpin fragments and comparable-length β-strand-loop-β-strand models, indicate 2-state folding for all topologies. N-terminal hairpin formation is the fold nucleating event for the wild-type sequence; the slower folding circular permutant has a more distributed folding transition state.

PubMed: 24350581
DOI: 10.1021/ja410824x

実験手法

SOLUTION NMR