2MCM

MACROMOMYCIN

Summary for 2MCM

• Entry DOI: 10.2210/pdb2mcm/pdb
• Descriptor: MACROMOMYCIN, CALCIUM ION, (4R)-2-METHYLPENTANE-2,4-DIOL, ... (5 entities in total)
• Functional Keywords: apoprotein
• Biological source: Streptomyces macromomyceticus
• Total number of polymer chains: 1
• Total formula weight: 11150.47

Authors

Van Roey, P. (deposition date: 1991-05-08, release date: 1993-01-15, Last modification date: 2024-10-30)

Primary citation

Van Roey, P.,Beerman, T.A.
Crystal structure analysis of auromomycin apoprotein (macromomycin) shows importance of protein side chains to chromophore binding selectivity.
Proc.Natl.Acad.Sci.Usa, 86:6587-6591, 1989

PubMed Abstract: The crystal structure of macromomycin, the apoprotein of the antitumor antibiotic auromomycin, has been determined and refined at 1.6-A resolution. The overall structure is composed of a flattened seven-stranded antiparallel beta-barrel and two antiparallel beta-sheet ribbons. The barrel and the ribbons define a deep cleft that is the chromophore binding site. The cleft is very accessible and in this structure is occupied by two 2-methyl-2,4-pentanediol and two water molecules. The overall shape of the binding site is similar to that of the analogue actinoxanthin. Highly specific side chains that are not conserved between different analogues extend into the binding site and may be important to the chromophore binding specificity.

PubMed: 2771945
DOI: 10.1073/pnas.86.17.6587

Experimental method

X-RAY DIFFRACTION (1.5 Å)