2MC6

A bacteriophage transcription regulator inhibits bacterial transcription initiation by sigma-factor displacement

Summary for 2MC6

• Entry DOI: 10.2210/pdb2mc6/pdb
• NMR Information: BMRB: 19429
• Descriptor: RNA polymerase inhibitor p7, DNA-directed RNA polymerase subunit beta' (2 entities in total)
• Functional Keywords: p7, rnap, sigma factor, beta prime subunit, ntd, transferase, transcription
• Biological source: Xanthomonas phage Xp10; More
• Total number of polymer chains: 2
• Total formula weight: 9425.43

Authors

Liu, B.,Shadrin, A.,Sheppard, C.,Xu, Y.,Severinov, K.,Matthews, S.,Wigneshweraraj, S. (deposition date: 2013-08-15, release date: 2014-04-02, Last modification date: 2024-05-01)

Primary citation

Liu, B.,Shadrin, A.,Sheppard, C.,Mekler, V.,Xu, Y.,Severinov, K.,Matthews, S.,Wigneshweraraj, S.
A bacteriophage transcription regulator inhibits bacterial transcription initiation by sigma-factor displacement.
Nucleic Acids Res., 42:4294-4305, 2014

PubMed Abstract: Bacteriophages (phages) appropriate essential processes of bacterial hosts to benefit their own development. The multisubunit bacterial RNA polymerase (RNAp) enzyme, which catalyses DNA transcription, is targeted by phage-encoded transcription regulators that selectively modulate its activity. Here, we describe the structural and mechanistic basis for the inhibition of bacterial RNAp by the transcription regulator P7 encoded by Xanthomonas oryzae phage Xp10. We reveal that P7 uses a two-step mechanism to simultaneously interact with the catalytic β and β' subunits of the bacterial RNAp and inhibits transcription initiation by inducing the displacement of the σ(70)-factor on initial engagement of RNAp with promoter DNA. The new mode of interaction with and inhibition mechanism of bacterial RNAp by P7 underscore the remarkable variety of mechanisms evolved by phages to interfere with host transcription.

PubMed: 24482445
DOI: 10.1093/nar/gku080

Experimental method

SOLUTION NMR