2MC5
A bacteriophage transcription regulator inhibits bacterial transcription initiation by -factor displacement
Summary for 2MC5
| Entry DOI | 10.2210/pdb2mc5/pdb |
| Related | 2MC6 |
| NMR Information | BMRB: 19428 |
| Descriptor | 45L (1 entity in total) |
| Functional Keywords | p7, rnap, sigma factor, protein binding |
| Biological source | Xanthomonas phage Xp10 |
| Total number of polymer chains | 1 |
| Total formula weight | 8188.97 |
| Authors | Liu, B.,Shadrin, A.,Sheppard, C.,Xu, Y.,Severinov, K.,Matthews, S.,Wigneshweraraj, S. (deposition date: 2013-08-14, release date: 2014-03-26, Last modification date: 2024-05-01) |
| Primary citation | Liu, B.,Shadrin, A.,Sheppard, C.,Mekler, V.,Xu, Y.,Severinov, K.,Matthews, S.,Wigneshweraraj, S. A bacteriophage transcription regulator inhibits bacterial transcription initiation by sigma-factor displacement. Nucleic Acids Res., 42:4294-4305, 2014 Cited by PubMed Abstract: Bacteriophages (phages) appropriate essential processes of bacterial hosts to benefit their own development. The multisubunit bacterial RNA polymerase (RNAp) enzyme, which catalyses DNA transcription, is targeted by phage-encoded transcription regulators that selectively modulate its activity. Here, we describe the structural and mechanistic basis for the inhibition of bacterial RNAp by the transcription regulator P7 encoded by Xanthomonas oryzae phage Xp10. We reveal that P7 uses a two-step mechanism to simultaneously interact with the catalytic β and β' subunits of the bacterial RNAp and inhibits transcription initiation by inducing the displacement of the σ(70)-factor on initial engagement of RNAp with promoter DNA. The new mode of interaction with and inhibition mechanism of bacterial RNAp by P7 underscore the remarkable variety of mechanisms evolved by phages to interfere with host transcription. PubMed: 24482445DOI: 10.1093/nar/gku080 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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