2MC5

A bacteriophage transcription regulator inhibits bacterial transcription initiation by -factor displacement

2MC5 の概要

• エントリーDOI: 10.2210/pdb2mc5/pdb
• 関連するPDBエントリー: 2MC6
• NMR情報: BMRB: 19428
• 分子名称: 45L (1 entity in total)
• 機能のキーワード: p7, rnap, sigma factor, protein binding
• 由来する生物種: Xanthomonas phage Xp10
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8188.97

構造登録者

Liu, B.,Shadrin, A.,Sheppard, C.,Xu, Y.,Severinov, K.,Matthews, S.,Wigneshweraraj, S. (登録日: 2013-08-14, 公開日: 2014-03-26, 最終更新日: 2024-05-01)

主引用文献

Liu, B.,Shadrin, A.,Sheppard, C.,Mekler, V.,Xu, Y.,Severinov, K.,Matthews, S.,Wigneshweraraj, S.
A bacteriophage transcription regulator inhibits bacterial transcription initiation by sigma-factor displacement.
Nucleic Acids Res., 42:4294-4305, 2014

PubMed Abstract: Bacteriophages (phages) appropriate essential processes of bacterial hosts to benefit their own development. The multisubunit bacterial RNA polymerase (RNAp) enzyme, which catalyses DNA transcription, is targeted by phage-encoded transcription regulators that selectively modulate its activity. Here, we describe the structural and mechanistic basis for the inhibition of bacterial RNAp by the transcription regulator P7 encoded by Xanthomonas oryzae phage Xp10. We reveal that P7 uses a two-step mechanism to simultaneously interact with the catalytic β and β' subunits of the bacterial RNAp and inhibits transcription initiation by inducing the displacement of the σ(70)-factor on initial engagement of RNAp with promoter DNA. The new mode of interaction with and inhibition mechanism of bacterial RNAp by P7 underscore the remarkable variety of mechanisms evolved by phages to interfere with host transcription.

PubMed: 24482445
DOI: 10.1093/nar/gku080

実験手法

SOLUTION NMR