2MBX
Structure, dynamics and stability of allergen cod parvalbumin Gad m 1 by solution and high-pressure NMR.
Summary for 2MBX
| Entry DOI | 10.2210/pdb2mbx/pdb |
| NMR Information | BMRB: 18113 |
| Descriptor | Parvalbumin beta, CALCIUM ION (2 entities in total) |
| Functional Keywords | allergen, parvalbumin, ef-hand, contractile protein |
| Biological source | Gadus morhua (Atlantic cod) |
| Total number of polymer chains | 1 |
| Total formula weight | 11645.20 |
| Authors | Moraes, A.H.,Ackerbauer, D.,Bublin, M.,Ferreira, F.,Almeida, F.C.L.,Breiteneder, H.,Valente, A. (deposition date: 2013-08-07, release date: 2014-08-20, Last modification date: 2024-05-15) |
| Primary citation | Moraes, A.H.,Ackerbauer, D.,Kostadinova, M.,Bublin, M.,de Oliveira, G.A.,Ferreira, F.,Almeida, F.C.,Breiteneder, H.,Valente, A.P. Solution and high-pressure NMR studies of the structure, dynamics, and stability of the cross-reactive allergenic cod parvalbumin Gad m 1. Proteins, 82:3032-3042, 2014 Cited by PubMed Abstract: Beta-parvalbumins from different fish species have been identified as the main elicitors of IgE-mediated reactions in fish-allergic individuals. Here, we report for the first time the NMR determination of the structure and dynamics of the major Atlantic cod (Gadus morhua) allergen Gad m 1 and compare them with other known parvalbumins. Although the Gad m 1 structure and accessibility of putative IgE epitopes are similar to parvalbumins in mackerel and carp, the charge distribution at the putative epitopes is different. The determination of the Gad m 1 structure contributes to a better understanding of cross-reactivity among fish parvalbumins. In addition, the high-pressure NMR and temperature variation experiments revealed the important contribution of the AB motif and other regions to the protein folding. This structural information could assist the future identification of hot spots for targeted mutations to develop hypoallergenic Ca(2+) -free forms for potential use in immunotherapy. PubMed: 25116395DOI: 10.1002/prot.24664 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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