2MBD

Lasiocepsin

2MBD の概要

• エントリーDOI: 10.2210/pdb2mbd/pdb
• NMR情報: BMRB: 19396
• 分子名称: lasiocepsin (1 entity in total)
• 機能のキーワード: antimicrobial peptide, wild bee, venom, antimicrobial protein
• 由来する生物種: Lasioglossum laticeps (bees)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2906.84

構造登録者

Monincova, L.,Budesinsky, M.,Cujova, S.,Cerovsky, V.,Veverka, V. (登録日: 2013-07-30, 公開日: 2014-01-15, 最終更新日: 2024-10-30)

主引用文献

Monincova, L.,Budesinsky, M.,Cujova, S.,Cerovsky, V.,Veverka, V.
Structural basis for antimicrobial activity of lasiocepsin.
Chembiochem, 15:301-308, 2014

PubMed Abstract: Lasiocepsin is a unique 27-residue antimicrobial peptide, isolated from Lasioglossum laticeps (wild bee) venom, with substantial antibacterial and antifungal activity. It adopts a well-defined structure consisting of two α-helices linked by a structured loop. Its basic residues form two distinct positively charged regions on the surface whereas aliphatic side chains contribute to solvent-accessible hydrophobic areas, thus emphasising the amphipathic character of the molecule. Lasiocepsin structurally belongs to the ShK family and shows a strong preference for anionic phospholipids; this is further augmented by increasing concentrations of cardiolipin, such as those found at the poles of bacterial cells. The membrane-permeabilising activity of the peptide is not limited to outer membranes of Gram-negative bacteria. The peptide interacts with phospholipids initially through its N terminus, and its degree of penetration is strongly dependent on the presence of cardiolipin.

PubMed: 24339323
DOI: 10.1002/cbic.201300509

実験手法

SOLUTION NMR