2MBB

Solution Structure of the human Polymerase iota UBM1-Ubiquitin Complex

2MBB の概要

• エントリーDOI: 10.2210/pdb2mbb/pdb
• NMR情報: BMRB: 19394
• 分子名称: Immunoglobulin G-binding protein G/DNA polymerase iota fusion protein, Polyubiquitin-B (2 entities in total)
• 機能のキーワード: polymerase iota, ubm, ubm1, ubiquitin, transferase, signaling protein
• 由来する生物種: Streptococcus sp. 'group G', Homo sapiens; 詳細
• 細胞内の位置: Nucleus: Q9UNA4; Ubiquitin: Cytoplasm (By similarity): P0CG47
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 20796.27

構造登録者

Wang, S.,Zhou, P. (登録日: 2013-07-29, 公開日: 2014-06-04, 最終更新日: 2024-05-15)

主引用文献

Wang, S.,Zhou, P.
Sparsely-sampled, high-resolution 4-D omit spectra for detection and assignment of intermolecular NOEs of protein complexes.
J.Biomol.Nmr, 59:51-56, 2014

PubMed Abstract: Unambiguous detection and assignment of intermolecular NOEs are essential for structure determination of protein complexes by NMR. Such information has traditionally been obtained with 3-D half-filtered experiments, where scalar coupling-based purging of intramolecular signals allows for selective detection of intermolecular NOEs. However, due to the large variation of (1)JHC scalar couplings and limited chemical shift dispersion in the indirect proton dimension, it is difficult to obtain reliable and complete assignments of interfacial NOEs. Here, we demonstrate a strategy that combines selective labeling and high-resolution 4-D NOE spectroscopy with sparse sampling for reliable identification and assignment of intermolecular NOEs. Spectral subtraction of component-labeled complexes from a uniformly-labeled protein complex yields an "omit" spectrum containing positive intermolecular NOEs with little signal degeneracy. Such a strategy can be broadly applied to unbiased detection, assignment and presentation of intermolecular NOEs of protein complexes.

PubMed: 24789524
DOI: 10.1007/s10858-014-9834-2

実験手法

SOLUTION NMR