2MB4

Solution structure of a stacked dimeric G-quadruplex formed by a segment of the human CEB1 minisatellite

2MB4 の概要

• エントリーDOI: 10.2210/pdb2mb4/pdb
• NMR情報: BMRB: 19389
• 分子名称: DNA_(5'-D(*AP*GP*GP*GP*GP*GP*GP*AP*GP*GP*GP*AP*GP*GP*GP*TP*GP*G)-3') (1 entity in total)
• 機能のキーワード: g-quadruplex, stacking, ceb1, minisatellite, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 11615.48

構造登録者

Adrian, M.,Ang, D.J.,Lech, C.,Heddi, B.,Nicolas, A.,Phan, A.T. (登録日: 2013-07-25, 公開日: 2014-05-28, 最終更新日: 2024-05-15)

主引用文献

Adrian, M.,Ang, D.J.,Lech, C.J.,Heddi, B.,Nicolas, A.,Phan, A.T.
Structure and Conformational Dynamics of a Stacked Dimeric G-Quadruplex Formed by the Human CEB1 Minisatellite.
J.Am.Chem.Soc., 136:6297-6305, 2014

PubMed Abstract: CEB1 is a highly polymorphic human minisatellite. In yeast, the size variation of CEB1 tandem arrays has been associated with the capacity of the motif to form G-quadruplexes. Here we report on the NMR solution structure of a G-quadruplex formed by the CEB1 DNA G-rich fragment d(AGGGGGGAGGGAGGGTGG), harboring several G-tracts including one with six continuous guanines. This sequence forms a dimeric G-quadruplex involving the stacking of two subunits, each being a unique snapback parallel-stranded scaffold with three G-tetrad layers, three double-chain-reversal loops, and a V-shaped loop. The two subunits are stacked at their 5'-end tetrads, and multiple stacking rotamers may be present due to a high symmetry at the stacking interface. There is a conformational exchange in the millisecond time scale involving a swapping motion between two bases of the six-guanine tract. Our results not only add to the understanding of how the G-quadruplex formation in human minisatellite leads to genetic instability but also address the fundamental questions regarding stacking of G-quadruplexes and how a long continuous G-tract participates in the structure and conformational dynamics of G-quadruplexes.

PubMed: 24742225
DOI: 10.1021/ja4125274

実験手法

SOLUTION NMR