2MAP

Solution structure of the complex formed by the region 2 of E. coli sigmaE and its cognate -10 promoter element non template strand TGTCAAA.

Summary for 2MAP

• Entry DOI: 10.2210/pdb2map/pdb
• Related: 2mao
• NMR Information: BMRB: 19367
• Descriptor: RNA polymerase sigma factor, DNA (2 entities in total)
• Functional Keywords: ecf sigma factor, -10 element recognition, protein-ssdna complex, transcription-dna complex, transcription/dna
• Biological source: Escherichia coli
• Total number of polymer chains: 2
• Total formula weight: 13146.02

Authors

Campagne, S.,Vorholt, J.A.,Allain, F.H.-T. (deposition date: 2013-07-16, release date: 2014-02-19, Last modification date: 2024-05-15)

Primary citation

Campagne, S.,Marsh, M.E.,Capitani, G.,Vorholt, J.A.,Allain, F.H.
Structural basis for -10 promoter element melting by environmentally induced sigma factors.
Nat.Struct.Mol.Biol., 21:269-276, 2014

PubMed Abstract: Bacterial transcription is controlled by sigma factors, the RNA polymerase subunits that act as initiation factors. Although a single housekeeping sigma factor enables transcription from thousands of promoters, environmentally induced sigma factors redirect gene expression toward small regulons to carry out focused responses. Using structural and functional analyses, we determined the molecular basis of -10 promoter element recognition by Escherichia coli σ(E), which revealed an unprecedented way to achieve promoter melting. Group IV sigma factors induced strand separation at the -10 element by flipping out a single nucleotide from the nontemplate-strand DNA base stack. Unambiguous selection of this critical base was driven by a dynamic protein loop, which can be substituted to modify specificity of promoter recognition. This mechanism of promoter melting explains the increased promoter-selection stringency of environmentally induced sigma factors.

PubMed: 24531660
DOI: 10.1038/nsmb.2777

Experimental method

SOLUTION NMR