2MAP

Solution structure of the complex formed by the region 2 of E. coli sigmaE and its cognate -10 promoter element non template strand TGTCAAA.

2MAP の概要

• エントリーDOI: 10.2210/pdb2map/pdb
• 関連するPDBエントリー: 2mao
• NMR情報: BMRB: 19367
• 分子名称: RNA polymerase sigma factor, DNA (2 entities in total)
• 機能のキーワード: ecf sigma factor, -10 element recognition, protein-ssdna complex, transcription-dna complex, transcription/dna
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 13146.02

構造登録者

Campagne, S.,Vorholt, J.A.,Allain, F.H.-T. (登録日: 2013-07-16, 公開日: 2014-02-19, 最終更新日: 2024-05-15)

主引用文献

Campagne, S.,Marsh, M.E.,Capitani, G.,Vorholt, J.A.,Allain, F.H.
Structural basis for -10 promoter element melting by environmentally induced sigma factors.
Nat.Struct.Mol.Biol., 21:269-276, 2014

PubMed Abstract: Bacterial transcription is controlled by sigma factors, the RNA polymerase subunits that act as initiation factors. Although a single housekeeping sigma factor enables transcription from thousands of promoters, environmentally induced sigma factors redirect gene expression toward small regulons to carry out focused responses. Using structural and functional analyses, we determined the molecular basis of -10 promoter element recognition by Escherichia coli σ(E), which revealed an unprecedented way to achieve promoter melting. Group IV sigma factors induced strand separation at the -10 element by flipping out a single nucleotide from the nontemplate-strand DNA base stack. Unambiguous selection of this critical base was driven by a dynamic protein loop, which can be substituted to modify specificity of promoter recognition. This mechanism of promoter melting explains the increased promoter-selection stringency of environmentally induced sigma factors.

PubMed: 24531660
DOI: 10.1038/nsmb.2777

実験手法

SOLUTION NMR