2MAL

Solution structure of Lipid Transfer Protein from Lentil Lens Culinaris

「2LJO」から置き換えられました

2MAL の概要

• エントリーDOI: 10.2210/pdb2mal/pdb
• 関連するPDBエントリー: 1AFH 1BV2 1BWO 1CZ2 1GH1 1LIP 1RZL 1SIY 1T12 1UVA 1UVB 1UVC
• NMR情報: BMRB: 19365
• 分子名称: Non-specific lipid-transfer protein 2 (1 entity in total)
• 機能のキーワード: plant lipid transfer protein, lens culinaris, lipid transport
• 由来する生物種: Lens culinaris (Lentil)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9299.69

構造登録者

Gizatullina, A.K.,Mineev, K.S.,Shenkarev, Z.O. (登録日: 2013-07-16, 公開日: 2013-10-02, 最終更新日: 2024-11-20)

主引用文献

Gizatullina, A.K.,Finkina, E.I.,Mineev, K.S.,Melnikova, D.N.,Bogdanov, I.V.,Telezhinskaya, I.N.,Balandin, S.V.,Shenkarev, Z.O.,Arseniev, A.S.,Ovchinnikova, T.V.
Recombinant production and solution structure of lipid transfer protein from lentil Lens culinaris.
Biochem.Biophys.Res.Commun., 439:427-432, 2013

PubMed Abstract: Lipid transfer protein, designated as Lc-LTP2, was isolated from seeds of the lentil Lens culinaris. The protein has molecular mass 9282.7Da, consists of 93 amino acid residues including 8 cysteines forming 4 disulfide bonds. Lc-LTP2 and its stable isotope labeled analogues were overexpressed in Escherichia coli and purified. Antimicrobial activity of the recombinant protein was examined, and its spatial structure was studied by NMR spectroscopy. The polypeptide chain of Lc-LTP2 forms four α-helices (Cys4-Leu18, Pro26-Ala37, Thr42-Ala56, Thr64-Lys73) and a long C-terminal tail without regular secondary structure. Side chains of the hydrophobic residues form a relatively large internal tunnel-like lipid-binding cavity (van der Waals volume comes up to ∼600Å(3)). The side-chains of Arg45, Pro79, and Tyr80 are located near an assumed mouth of the cavity. Titration with dimyristoyl phosphatidylglycerol (DMPG) revealed formation of the Lc-LTP2/lipid non-covalent complex accompanied by rearrangements in the protein spatial structure and expansion of the internal cavity. The resultant Lc-LTP2/DMPG complex demonstrates limited lifetime and dissociates within tens of hours.

PubMed: 23998937
DOI: 10.1016/j.bbrc.2013.08.078

実験手法

SOLUTION NMR