2M8U

Solution structure of the Dictyostelium discodieum Myosin Light Chain, MlcC

2M8U の概要

• エントリーDOI: 10.2210/pdb2m8u/pdb
• NMR情報: BMRB: 19274
• 分子名称: Myosin Light Chain, MlcC (1 entity in total)
• 機能のキーワード: myosin light chain, helical bundle, contractile protein
• 由来する生物種: Dictyostelium discoideum (Slime mold)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8917.95

構造登録者

Liburd, J.D.,Miller, E.,Langelaan, D.,Chitayat, S.,Crawley, S.W.,Cote, G.P.,Smith, S.P. (登録日: 2013-05-28, 公開日: 2014-12-24, 最終更新日: 2024-05-15)

主引用文献

Langelaan, D.N.,Liburd, J.,Yang, Y.,Miller, E.,Chitayat, S.,Crawley, S.W.,Cote, G.P.,Smith, S.P.
Structure of the Single-lobe Myosin Light Chain C in Complex with the Light Chain-binding Domains of Myosin-1C Provides Insights into Divergent IQ Motif Recognition.
J.Biol.Chem., 291:19607-19617, 2016

PubMed Abstract: Myosin light chains are key regulators of class 1 myosins and typically comprise two domains, with calmodulin being the archetypal example. They bind IQ motifs within the myosin neck region and amplify conformational changes in the motor domain. A single lobe light chain, myosin light chain C (MlcC), was recently identified and shown to specifically bind to two sequentially divergent IQ motifs of the Dictyostelium myosin-1C. To provide a molecular basis of this interaction, the structures of apo-MlcC and a 2:1 MlcC·myosin-1C neck complex were determined. The two non-functional EF-hand motifs of MlcC pack together to form a globular four-helix bundle that opens up to expose a central hydrophobic groove, which interacts with the N-terminal portion of the divergent IQ1 and IQ2 motifs. The N- and C-terminal regions of MlcC make critical contacts that contribute to its specific interactions with the myosin-1C divergent IQ motifs, which are contacts that deviate from the traditional mode of calmodulin-IQ recognition.

PubMed: 27466369
DOI: 10.1074/jbc.M116.746313

実験手法

SOLUTION NMR