2M8K

A pyrimidine motif triple helix in the Kluyveromyces lactis telomerase RNA pseudoknot is essential for function in vivo

2M8K の概要

• エントリーDOI: 10.2210/pdb2m8k/pdb
• NMR情報: BMRB: 19260
• 分子名称: RNA (48-MER) (1 entity in total)
• 機能のキーワード: telomerase, pseudoknot, rna triplex, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15152.86

構造登録者

Cash, D.D.,Cohen, O.,Kim, N.,Shefer, K.,Brown, Y.,Ulyanov, N.B.,Tzfati, Y.,Feigon, J. (登録日: 2013-05-22, 公開日: 2013-06-19, 最終更新日: 2024-05-15)

主引用文献

Cash, D.D.,Cohen-Zontag, O.,Kim, N.K.,Shefer, K.,Brown, Y.,Ulyanov, N.B.,Tzfati, Y.,Feigon, J.
Pyrimidine motif triple helix in the Kluyveromyces lactis telomerase RNA pseudoknot is essential for function in vivo.
Proc.Natl.Acad.Sci.USA, 110:10970-10975, 2013

PubMed Abstract: Telomerase is a ribonucleoprotein complex that extends the 3' ends of linear chromosomes. The specialized telomerase reverse transcriptase requires a multidomain RNA (telomerase RNA, TER), which includes an integral RNA template and functionally important template-adjacent pseudoknot. The structure of the human TER pseudoknot revealed that the loops interact with the stems to form a triple helix shown to be important for activity in vitro. A similar triple helix has been predicted to form in diverse fungi TER pseudoknots. The solution NMR structure of the Kluyveromyces lactis pseudoknot, presented here, reveals that it contains a long pyrimidine motif triple helix with unexpected features that include three individual bulge nucleotides and a C(+)•G-C triple adjacent to a stem 2-loop 2 junction. Despite significant differences in sequence and base triples, the 3D shape of the human and K. lactis TER pseudoknots are remarkably similar. Analysis of the effects of nucleotide substitutions on cell growth and telomere lengths provides evidence that this conserved structure forms in endogenously assembled telomerase and is essential for telomerase function in vivo.

PubMed: 23776224
DOI: 10.1073/pnas.1309590110

実験手法

SOLUTION NMR