2M87

Structural Basis of DNA Recognition by the Effector Domain of Klebsiella pneumoniae PmrA

2M87 の概要

• エントリーDOI: 10.2210/pdb2m87/pdb
• NMR情報: BMRB: 19231
• 分子名称: Transcriptional regulatory protein basR/pmrA (1 entity in total)
• 機能のキーワード: pmra, two-component signaling system, effector domain, dna-binding, signaling protein
• 由来する生物種: Klebsiella pneumoniae
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11854.45

構造登録者

Wang, I.,Lou, Y.C.,Chen, C. (登録日: 2013-05-07, 公開日: 2014-01-22, 最終更新日: 2024-05-15)

主引用文献

Lou, Y.C.,Wang, I.,Rajasekaran, M.,Kao, Y.F.,Ho, M.R.,Hsu, S.T.,Chou, S.H.,Wu, S.H.,Chen, C.
Solution structure and tandem DNA recognition of the C-terminal effector domain of PmrA from Klebsiella pneumoniae.
Nucleic Acids Res., 42:4080-4093, 2014

PubMed Abstract: Klebsiella pneumoniae PmrA is a polymyxin-resistance-associated response regulator. The C-terminal effector/DNA-binding domain of PmrA (PmrAC) recognizes tandem imperfect repeat sequences on the promoters of genes to induce antimicrobial peptide resistance after phosphorylation and dimerization of its N-terminal receiver domain (PmrAN). However, structural information concerning how phosphorylation of the response regulator enhances DNA recognition remains elusive. To gain insights, we determined the nuclear magnetic resonance solution structure of PmrAC and characterized the interactions between PmrAC or BeF3(-)-activated full-length PmrA (PmrAF) and two DNA sequences from the pbgP promoter of K. pneumoniae. We showed that PmrAC binds to the PmrA box, which was verified to contain two half-sites, 5'-CTTAAT-3' and 5'-CCTAAG-3', in a head-to-tail fashion with much stronger affinity to the first than the second site without cooperativity. The structural basis for the PmrAC-DNA complex was investigated using HADDOCK docking and confirmed by paramagnetic relaxation enhancement. Unlike PmrAC, PmrAF recognizes the two sites simultaneously and specifically. In the PmrAF-DNA complex, PmrAN may maintain an activated homodimeric conformation analogous to that in the free form and the interactions between two PmrAC molecules aid in bending and binding of the DNA duplex for transcription activation.

PubMed: 24371275
DOI: 10.1093/nar/gkt1345

実験手法

SOLUTION NMR