2M7G

Structure of the Type IVa Major Pilin from the Electrically Conductive Bacterial Nanowires of Geobacter sulfurreducens

Summary for 2M7G

• Entry DOI: 10.2210/pdb2m7g/pdb
• NMR Information: BMRB: 19185
• Descriptor: Geopilin domain 1 protein (1 entity in total)
• Functional Keywords: pilin, pila, bacterial nanowire, type iva, cell adhesion, structural protein, electron transport
• Biological source: Geobacter sulfurreducens
• Total number of polymer chains: 1
• Total formula weight: 6576.50

Authors

Reardon, P.N.,Mueller, K.T. (deposition date: 2013-04-22, release date: 2013-08-28, Last modification date: 2024-05-15)

Primary citation

Reardon, P.N.,Mueller, K.T.
Structure of the Type IVa Major Pilin from the Electrically Conductive Bacterial Nanowires of Geobacter sulfurreducens.
J.Biol.Chem., 288:29260-29266, 2013

PubMed Abstract: Several species of δ proteobacteria are capable of reducing insoluble metal oxides as well as other extracellular electron acceptors. These bacteria play a critical role in the cycling of minerals in subsurface environments, sediments, and groundwater. In some species of bacteria such as Geobacter sulfurreducens, the transport of electrons is proposed to be facilitated by filamentous fibers that are referred to as bacterial nanowires. These nanowires are polymeric assemblies of proteins belonging to the type IVa family of pilin proteins and are mainly comprised of one subunit protein, PilA. Here, we report the high resolution solution NMR structure of the PilA protein from G. sulfurreducens determined in detergent micelles. The protein is >85% α-helical and exhibits similar architecture to the N-terminal regions of other non-conductive type IVa pilins. The detergent micelle interacts with the first 21 amino acids of the protein, indicating that this region likely associates with the bacterial inner membrane prior to fiber formation. A model of the G. sulfurreducens pilus fiber is proposed based on docking of this structure into the fiber model of the type IVa pilin from Neisseria gonorrhoeae. This model provides insight into the organization of aromatic amino acids that are important for electrical conduction.

PubMed: 23965997
DOI: 10.1074/jbc.M113.498527

Experimental method

SOLUTION NMR