2M5U

NMR structure of the P4 hairpin of the CPEB3 ribozyme

2M5U の概要

• エントリーDOI: 10.2210/pdb2m5u/pdb
• NMR情報: BMRB: 19081
• 分子名称: RNA_(5'-R(*GP*GP*CP*AP*GP*AP*UP*UP*CP*UP*GP*GP*UP*GP*AP*AP*UP*CP*UP*GP*CP*C)-3') (1 entity in total)
• 機能のキーワード: rna, cpeb3, uggu tetraloop, ribozyme, hairpin
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7051.21

構造登録者

Skilandat, M.,Rowinska-Zyrek, M.,Sigel, R.K.O. (登録日: 2013-03-08, 公開日: 2014-04-02, 最終更新日: 2024-05-15)

主引用文献

Skilandat, M.,Rowinska-Zyrek, M.,Sigel, R.K.
Solution structure and metal ion binding sites of the human CPEB3 ribozyme's P4 domain.
J.Biol.Inorg.Chem., 19:903-912, 2014

PubMed Abstract: Three ribozymes are known to occur in humans, the CPEB3 ribozyme, the CoTC ribozyme, and the hammerhead ribozyme. Here, we present the NMR solution structure of a well-conserved motif within the CPEB3 ribozyme, the P4 domain. In addition, we discuss the binding sites and impact of Mg(2+) and [Co(NH3)6](3+), a spectroscopic probe for [Mg(H2O)6](2+), on the structure. The well-defined P4 region is a hairpin closed with a UGGU tetraloop that shows a distinct electrostatic surface potential and a characteristic, strongly curved backbone trajectory. The P4 hairpin contains two specific Mg(2+) binding sites: one outer-sphere binding site close to the proposed CPEB3 ribozyme active site with potential relevance for maintaining a compact fold of the ribozyme core, and one inner-sphere binding site, probably stabilizing the tetraloop structure. The structure of the tetraloop resembles an RNase III recognition structure, as previously described for an AGUU tetraloop. The detailed knowledge of the P4 domain and its metal ion binding preferences thus brings us closer to understanding the importance of Mg(2+) binding for the CPEB3 ribozyme's fold and function in the cell.

PubMed: 24652468
DOI: 10.1007/s00775-014-1125-6

実験手法

SOLUTION NMR