2M57

NMR solution structure of domain 5 from Azotobacter vinelandii Intron 5 at pH 7.8

2M57 の概要

• エントリーDOI: 10.2210/pdb2m57/pdb
• NMR情報: BMRB: 19039
• 分子名称: RNA_(35-MER) (1 entity in total)
• 機能のキーワード: rna, ribozyme, group ii intron, hairpin
• 由来する生物種: Azotobacter vinelandii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11268.71

構造登録者

Pechlaner, M.,Donghi, D.,Zelenay, V.,Sigel, R.K.O. (登録日: 2013-02-15, 公開日: 2014-02-26, 最終更新日: 2025-03-12)

主引用文献

Pechlaner, M.,Donghi, D.,Zelenay, V.,Sigel, R.K.
Protonation-Dependent Base Flipping at Neutral pH in the Catalytic Triad of a Self-Splicing Bacterial Group II Intron.
Angew.Chem.Int.Ed.Engl., 54:9687-9690, 2015

PubMed Abstract: NMR spectroscopy has revealed pH-dependent structural changes in the highly conserved catalytic domain 5 of a bacterial group II intron. Two adenines with pK(a) values close to neutral pH were identified in the catalytic triad and the bulge. Protonation of the adenine opposite to the catalytic triad is stabilized within a G(syn)-AH(+) (anti) base pair. The pH-dependent anti-to-syn flipping of this G in the catalytic triad modulates the known interaction with the linker region between domains 2 and 3 (J23) and simultaneously the binding of the catalytic Mg(2+) ion to its backbone. Hence, this here identified shifted pK(a) value controls the conformational change between the two steps of splicing.

PubMed: 26119804
DOI: 10.1002/anie.201504014

実験手法

SOLUTION NMR