2M3K

Global fold of the type IV pilin ComP from Neisseria meningitidis

2M3K の概要

• エントリーDOI: 10.2210/pdb2m3k/pdb
• NMR情報: BMRB: 18966
• 分子名称: Minor pilin ComP (1 entity in total)
• 機能のキーワード: dna uptake, motor protein
• 由来する生物種: Neisseria meningitidis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13512.77

構造登録者

Simpson, P. (登録日: 2013-01-21, 公開日: 2013-02-13, 最終更新日: 2024-10-16)

主引用文献

Cehovin, A.,Simpson, P.J.,McDowell, M.A.,Brown, D.R.,Noschese, R.,Pallett, M.,Brady, J.,Baldwin, G.S.,Lea, S.M.,Matthews, S.J.,Pelicic, V.
Specific DNA recognition mediated by a type IV pilin.
Proc.Natl.Acad.Sci.USA, 110:3065-3070, 2013

PubMed Abstract: Natural transformation is a dominant force in bacterial evolution by promoting horizontal gene transfer. This process may have devastating consequences, such as the spread of antibiotic resistance or the emergence of highly virulent clones. However, uptake and recombination of foreign DNA are most often deleterious to competent species. Therefore, model naturally transformable gram-negative bacteria, including the human pathogen Neisseria meningitidis, have evolved means to preferentially take up homotypic DNA containing short and genus-specific sequence motifs. Despite decades of intense investigations, the DNA uptake sequence receptor in Neisseria species has remained elusive. We show here, using a multidisciplinary approach combining biochemistry, molecular genetics, and structural biology, that meningococcal type IV pili bind DNA through the minor pilin ComP via an electropositive stripe that is predicted to be exposed on the filaments surface and that ComP displays an exquisite binding preference for DNA uptake sequence. Our findings illuminate the earliest step in natural transformation, reveal an unconventional mechanism for DNA binding, and suggest that selective DNA uptake is more widespread than previously thought.

PubMed: 23386723
DOI: 10.1073/pnas.1218832110

実験手法

SOLUTION NMR