2M37

Structure of lasso peptide astexin-1

Summary for 2M37

• Entry DOI: 10.2210/pdb2m37/pdb
• NMR Information: BMRB: 18948
• Descriptor: ASTEXIN-1 (1 entity in total)
• Functional Keywords: astexin-1, lasso peptide, lariat protoknot, unknown function
• Biological source: Asticcacaulis excentricus
• Total number of polymer chains: 1
• Total formula weight: 2113.22

Authors

Zimmermann, M.,Hegemann, J.D.,Xie, X.,Marahiel, M.A. (deposition date: 2013-01-14, release date: 2013-05-01, Last modification date: 2024-11-06)

Primary citation

Zimmermann, M.,Hegemann, J.D.,Xie, X.,Marahiel, M.A.
The astexin-1 lasso peptides: biosynthesis, stability, and structural studies.
Chem.Biol., 20:558-569, 2013

PubMed Abstract: Lasso peptides are a large family of natural products that owe their name to a unique structure formed by a side chain to backbone macrocyclization, resembling a knotted lasso. The unique structure has significant impact on their biological and physical properties, as lasso peptides are usually more stable than linear ones. Current work examines stability, structure, and biosynthesis of recently discovered lasso peptide astexin-1, a heat-sensitive lasso peptide. The obtained results revealed a new lasso structure with a tight loop and long tail as well as narrow specificity of the maturation machinery for some essential residues associated with the protease processing site, involved in macrolactam ring formation and entrapment of the tail. Using the astexin-1 structure, it was possible to rationally construct a thermostable variant of this lasso peptide.

PubMed: 23601645
DOI: 10.1016/j.chembiol.2013.03.013

Experimental method

SOLUTION NMR