2M37

Structure of lasso peptide astexin-1

2M37 の概要

• エントリーDOI: 10.2210/pdb2m37/pdb
• NMR情報: BMRB: 18948
• 分子名称: ASTEXIN-1 (1 entity in total)
• 機能のキーワード: astexin-1, lasso peptide, lariat protoknot, unknown function
• 由来する生物種: Asticcacaulis excentricus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2113.22

構造登録者

Zimmermann, M.,Hegemann, J.D.,Xie, X.,Marahiel, M.A. (登録日: 2013-01-14, 公開日: 2013-05-01, 最終更新日: 2024-11-06)

主引用文献

Zimmermann, M.,Hegemann, J.D.,Xie, X.,Marahiel, M.A.
The astexin-1 lasso peptides: biosynthesis, stability, and structural studies.
Chem.Biol., 20:558-569, 2013

PubMed Abstract: Lasso peptides are a large family of natural products that owe their name to a unique structure formed by a side chain to backbone macrocyclization, resembling a knotted lasso. The unique structure has significant impact on their biological and physical properties, as lasso peptides are usually more stable than linear ones. Current work examines stability, structure, and biosynthesis of recently discovered lasso peptide astexin-1, a heat-sensitive lasso peptide. The obtained results revealed a new lasso structure with a tight loop and long tail as well as narrow specificity of the maturation machinery for some essential residues associated with the protease processing site, involved in macrolactam ring formation and entrapment of the tail. Using the astexin-1 structure, it was possible to rationally construct a thermostable variant of this lasso peptide.

PubMed: 23601645
DOI: 10.1016/j.chembiol.2013.03.013

実験手法

SOLUTION NMR