2M2C

Solution structure of Duplex DNA

Summary for 2M2C

• Entry DOI: 10.2210/pdb2m2c/pdb
• NMR Information: BMRB: 18907
• Descriptor: DNA (5'-D(*GP*CP*GP*CP*AP*TP*GP*CP*TP*AP*CP*GP*CP*G)-3'), DNA (5'-D(*CP*GP*CP*GP*TP*AP*GP*CP*AP*TP*GP*CP*GP*C)-3') (2 entities in total)
• Functional Keywords: duplex dna, gg28, dna
• Biological source: Synthetic DNA; More
• Total number of polymer chains: 2
• Total formula weight: 8563.56

Authors

Ghosh, A.,Kar, R.K.,Chatterjee, S.,Bhunia, A. (deposition date: 2012-12-18, release date: 2013-01-23, Last modification date: 2024-05-15)

Primary citation

Ghosh, A.,Kar, R.K.,Jana, J.,Saha, A.,Jana, B.,Krishnamoorthy, J.,Kumar, D.,Ghosh, S.,Chatterjee, S.,Bhunia, A.
Indolicidin targets duplex DNA: structural and mechanistic insight through a combination of spectroscopy and microscopy.
Chemmedchem, 9:2052-2058, 2014

PubMed Abstract: Indolicidin (IR13), a 13-residue antimicrobial peptide from the cathelicidin family, is known to exhibit a broad spectrum of antimicrobial activity against various microorganisms. This peptide inhibits bacterial DNA synthesis resulting in cell filamentation. However, the precise mechanism remains unclear and requires further investigation. The central PWWP motif of IR13 provides a unique structural element that can wrap around, and thus stabilize, duplex B-type DNA structures. Replacements of the central Trp-Trp pair with Ala-Ala, His-His, or Phe-Phe residues in the PxxP motif significantly affects the ability of the peptide to stabilize duplex DNA. Results of microscopy studies in conjunction with spectroscopic data confirm that the DNA duplex is stabilized by IR13, thereby inhibiting DNA replication and transcription. In this study we provide high-resolution structural information on the interaction between indolicidin and DNA, which will be beneficial for the design of novel therapeutic antibiotics based on peptide scaffolds.

PubMed: 25044630
DOI: 10.1002/cmdc.201402215

Experimental method

SOLUTION NMR