2M1U

Solution structure of the small dictyostelium discoideium myosin light chain mlcb provides insights into iq-motif recognition of class i myosin myo1b

2M1U の概要

• エントリーDOI: 10.2210/pdb2m1u/pdb
• NMR情報: BMRB: 18880
• 分子名称: myosin light chain MlcB (1 entity in total)
• 機能のキーワード: myosin light chain, contractile protein
• 由来する生物種: Saccharomyces cerevisiae
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10471.38

構造登録者

Liburd, J.,Chitayat, S.,Crawley, S.W.,Denis, C.M.,Cote, G.P.,Smith, S.P. (登録日: 2012-12-06, 公開日: 2013-12-11, 最終更新日: 2024-05-15)

主引用文献

Liburd, J.,Chitayat, S.,Crawley, S.W.,Munro, K.,Miller, E.,Denis, C.M.,Spencer, H.L.,Cote, G.P.,Smith, S.P.
Structure of the small Dictyostelium discoideum myosin light chain MlcB provides insights into MyoB IQ motif recognition.
J.Biol.Chem., 289:17030-17042, 2014

PubMed Abstract: Dictyostelium discoideum MyoB is a class I myosin involved in the formation and retraction of membrane projections, cortical tension generation, membrane recycling, and phagosome maturation. The MyoB-specific, single-lobe EF-hand light chain MlcB binds the sole IQ motif of MyoB with submicromolar affinity in the absence and presence of Ca(2+). However, the structural features of this novel myosin light chain and its interaction with its cognate IQ motif remain uncharacterized. Here, we describe the NMR-derived solution structure of apoMlcB, which displays a globular four-helix bundle. Helix 1 adopts a unique orientation when compared with the apo states of the EF-hand calcium-binding proteins calmodulin, S100B, and calbindin D9k. NMR-based chemical shift perturbation mapping identified a hydrophobic MyoB IQ binding surface that involves amino acid residues in helices I and IV and the functional N-terminal Ca(2+) binding loop, a site that appears to be maintained when MlcB adopts the holo state. Complementary mutagenesis and binding studies indicated that residues Ile-701, Phe-705, and Trp-708 of the MyoB IQ motif are critical for recognition of MlcB, which together allowed the generation of a structural model of the apoMlcB-MyoB IQ complex. We conclude that the mode of IQ motif recognition by the novel single-lobe MlcB differs considerably from that of stereotypical bilobal light chains such as calmodulin.

PubMed: 24790102
DOI: 10.1074/jbc.M113.536532

実験手法

SOLUTION NMR