2M1G

Parallel human telomeric quadruplex containing 2'F-ANA substitutions

Summary for 2M1G

• Entry DOI: 10.2210/pdb2m1g/pdb
• NMR Information: BMRB: 18862
• Descriptor: 5'-D(*TP*AP*GP*GP*GP*TP*TP*AP*(GFL)P*GP*GP*T)-3' (1 entity in total)
• Functional Keywords: parallel g-quadruplex, human telomeric sequence, fluoro-arabinose, molecular interactions, dna
• Total number of polymer chains: 2
• Total formula weight: 7582.91

Authors

Martin-Pintado, N.,Yahyaee-Anzahaee, M.,Deleavey, G.F.,Portella, G.,Orozco, M.,Damha, M.J.,Gonzalez, C. (deposition date: 2012-11-27, release date: 2013-05-08, Last modification date: 2024-05-01)

Primary citation

Martin-Pintado, N.,Yahyaee-Anzahaee, M.,Deleavey, G.F.,Portella, G.,Orozco, M.,Damha, M.J.,Gonzalez, C.
Dramatic effect of furanose c2' substitution on structure and stability: directing the folding of the human telomeric quadruplex with a single fluorine atom.
J.Am.Chem.Soc., 135:5344-5347, 2013

PubMed Abstract: Human telomeric DNA quadruplexes can adopt different conformations in solution. We have found that arabinose, 2'F-arabinose, and ribose substitutions stabilize the propeller parallel G-quadruplex form over competing conformers, allowing NMR structural determination of this particularly significant nucleic acid structure. 2'F-arabinose substitution provides the greatest stabilization as a result of electrostatic (F-CH---O4') and pseudo-hydrogen-bond (F---H8) stabilizing interactions. In contrast, 2'F-rG substitution provokes a dramatic destabilization of the quadruplex structure due to unfavorable electrostatic repulsion between the phosphate and the 2'-F.

PubMed: 23521511
DOI: 10.1021/ja401954t

Experimental method

SOLUTION NMR