2M0R

Solution structure and dynamics of human S100A14

Summary for 2M0R

• Entry DOI: 10.2210/pdb2m0r/pdb
• NMR Information: BMRB: 18818
• Descriptor: Protein S100-A14 (1 entity in total)
• Functional Keywords: ef-hand proteins, protein dynamics, metal binding protein
• Biological source: Homo sapiens (human)
• Cellular location: Cytoplasm: Q9HCY8
• Total number of polymer chains: 2
• Total formula weight: 23354.17

Authors

Bertini, I.,Borsi, V.,Cerofolini, L.,Das Gupta, S.,Fragai, M.,Luchinat, C. (deposition date: 2012-11-05, release date: 2013-01-23, Last modification date: 2024-05-15)

Primary citation

Bertini, I.,Borsi, V.,Cerofolini, L.,Das Gupta, S.,Fragai, M.,Luchinat, C.
Solution structure and dynamics of human S100A14.
J.Biol.Inorg.Chem., 18:183-194, 2013

PubMed Abstract: Human S100A14 is a member of the EF-hand calcium-binding protein family that has only recently been described in terms of its functional and pathological properties. The protein is overexpressed in a variety of tumor cells and it has been shown to trigger receptor for advanced glycation end products (RAGE)-dependent signaling in cell cultures. The solution structure of homodimeric S100A14 in the apo state has been solved at physiological temperature. It is shown that the protein does not bind calcium(II) ions and exhibits a "semi-open" conformation that thus represents the physiological structure of the S100A14. The lack of two ligands in the canonical EF-hand calcium(II)-binding site explains the negligible affinity for calcium(II) in solution, and the exposed cysteines and histidine account for the observed precipitation in the presence of zinc(II) or copper(II) ions.

PubMed: 23197251
DOI: 10.1007/s00775-012-0963-3

Experimental method

SOLUTION NMR