2LZI

High resolution NMR structure of the theta-defensin HTD-2 (retrocyclin 2)

2LZI の概要

• エントリーDOI: 10.2210/pdb2lzi/pdb
• 関連するPDBエントリー: 1HVZ 2ATG
• NMR情報: BMRB: 18757
• 分子名称: retrocyclin 2 (1 entity in total)
• 機能のキーワード: theta-defensin, cyclic peptides, cyclic cystine ladder, antiviral protein
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2046.63

構造登録者

Conibear, A.C.,Rosengren, K.,Harvey, P.J.,Craik, D.J. (登録日: 2012-10-02, 公開日: 2012-11-28, 最終更新日: 2024-11-20)

主引用文献

Conibear, A.C.,Rosengren, K.J.,Harvey, P.J.,Craik, D.J.
Structural characterization of the cyclic cystine ladder motif of theta-defensins.
Biochemistry, 51:9718-9726, 2012

PubMed Abstract: The θ-defensins are, to date, the only known ribosomally synthesized cyclic peptides in mammals, and they have promising antimicrobial bioactivities. The characteristic structural motif of the θ-defensins is the cyclic cystine ladder, comprising a cyclic peptide backbone and three parallel disulfide bonds. In contrast to the cyclic cystine knot, which characterizes the plant cyclotides, the cyclic cystine ladder has not been as well described as a structural motif. Here we report the solution structures and nuclear magnetic resonance relaxation properties in aqueous solution of three representative θ-defensins from different species. Our data suggest that the θ-defensins are more rigid and structurally defined than previously thought. In addition, all three θ-defensins were found to self-associate in aqueous solution in a concentration-dependent and reversible manner, a property that might have a role in their mechanism of action. The structural definition of the θ-defensins and the cyclic cystine ladder will help to guide exploitation of these molecules as structural frameworks for the design of peptide drugs.

PubMed: 23148585
DOI: 10.1021/bi301363a

実験手法

SOLUTION NMR