2LZF

Structure of the biofilm matrix promoter AbbA from B. subtilis

2LZF の概要

• エントリーDOI: 10.2210/pdb2lzf/pdb
• NMR情報: BMRB: 18753
• 分子名称: Uncharacterized protein ykzF (1 entity in total)
• 機能のキーワード: biofilm, dna-mimic, abrb, protein binding
• 由来する生物種: Bacillus subtilis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 15978.03

構造登録者

Bobay, B.G.,Tucker, A.T.,Losick, R.,Cavanagh, J. (登録日: 2012-10-02, 公開日: 2013-09-11, 最終更新日: 2024-05-15)

主引用文献

Tucker, A.T.,Bobay, B.G.,Banse, A.V.,Olson, A.L.,Soderblom, E.J.,Moseley, M.A.,Thompson, R.J.,Varney, K.M.,Losick, R.,Cavanagh, J.
A DNA Mimic: The Structure and Mechanism of Action for the Anti-Repressor Protein AbbA.
J.Mol.Biol., 426:1911-1924, 2014

PubMed Abstract: Bacteria respond to adverse environmental conditions by switching on the expression of large numbers of genes that enable them to adapt to unfavorable circumstances. In Bacillus subtilis, many adaptive genes are under the negative control of the global transition state regulator, the repressor protein AbrB. Stressful conditions lead to the de-repression of genes under AbrB control. Contributing to this de-repression is AbbA, an anti-repressor that binds to and blocks AbrB from binding to DNA. Here, we have determined the NMR structure of the functional AbbA dimer, confirmed that it binds to the N-terminal DNA-binding domain of AbrB, and have provided an initial description for the interaction using computational docking procedures. Interestingly, we show that AbbA has structural and surface characteristics that closely mimic the DNA phosphate backbone, enabling it to readily carry out its physiological function.

PubMed: 24534728
DOI: 10.1016/j.jmb.2014.02.010

実験手法

SOLUTION NMR