2LYG

Fuc_TBA

2LYG の概要

• エントリーDOI: 10.2210/pdb2lyg/pdb
• NMR情報: BMRB: 18724
• 分子名称: DNA (5'-D(P*GP*GP*TP*TP*GP*GP*TP*GP*TP*GP*GP*TP*TP*GP*G)-3'), 2-hydroxyethyl 6-deoxy-beta-L-galactopyranoside (2 entities in total)
• 機能のキーワード: carbohydrate-dna interaction, g-quadruplex structure, dna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4951.26

構造登録者

Gomez-Pinto, I.,Vengut-Climent, E.,Lucas, R.,Avio, A.,Eritja, R.,Gonzalez-Ibaez, C.,Morales, J. (登録日: 2012-09-18, 公開日: 2014-01-29, 最終更新日: 2024-05-01)

主引用文献

Gomez-Pinto, I.,Vengut-Climent, E.,Lucas, R.,Avino, A.,Eritja, R.,Gonzalez, C.,Morales, J.C.
Carbohydrate-DNA interactions at G-quadruplexes: folding and stability changes by attaching sugars at the 5'-end.
Chemistry, 19:1920-1927, 2013

PubMed Abstract: Quadruplex DNA structures are attracting an enormous interest in many areas of chemistry, ranging from chemical biology, supramolecular chemistry to nanoscience. We have prepared carbohydrate-DNA conjugates containing the oligonucleotide sequences of G-quadruplexes (thrombin binding aptamer (TBA) and human telomere (TEL)), measured their thermal stability and studied their structure in solution by using NMR and molecular dynamics. The solution structure of a fucose-TBA conjugate shows stacking interactions between the carbohydrate and the DNA G-tetrad in addition to hydrogen bonding and hydrophobic contacts. We have also shown that attaching carbohydrates at the 5'-end of a quadruplex telomeric sequence can alter its folding topology. These results suggest the possibility of modulating the folding of the G-quadruplex by linking carbohydrates and have clear implications in molecular recognition and the design of new G-quadruplex ligands.

PubMed: 23315826
DOI: 10.1002/chem.201203902

実験手法

SOLUTION NMR