2LY4

HMGB1-facilitated p53 DNA binding occurs via HMG-box/p53 transactivation domain interaction and is regulated by the acidic tail

2LY4 の概要

• エントリーDOI: 10.2210/pdb2ly4/pdb
• NMR情報: BMRB: 18709
• 分子名称: High mobility group protein B1, Cellular tumor antigen p53 (2 entities in total)
• 機能のキーワード: hmg, p53, tad, nuclear protein-antitumour protein complex, nuclear protein/antitumour protein
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus: P09429; Cytoplasm. Isoform 1: Nucleus. Isoform 2: Nucleus. Isoform 3: Nucleus. Isoform 4: Nucleus. Isoform 7: Nucleus. Isoform 8: Nucleus. Isoform 9: Cytoplasm: P04637
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 19686.19

構造登録者

Rowell, J.P.,Simpson, K.L.,Stott, K.,Watson, M.,Thomas, J.O. (登録日: 2012-09-12, 公開日: 2012-10-31, 最終更新日: 2024-05-01)

主引用文献

Rowell, J.P.,Simpson, K.L.,Stott, K.,Watson, M.,Thomas, J.O.
HMGB1-Facilitated p53 DNA Binding Occurs via HMG-Box/p53 Transactivation Domain Interaction, Regulated by the Acidic Tail.
Structure, 20:2014-2024, 2012

PubMed Abstract: Facilitated binding of p53 to DNA by high mobility group B1 (HMGB1) may involve interaction between the N-terminal region of p53 and the high mobility group (HMG) boxes, as well as HMG-induced bending of the DNA. Intramolecular shielding of the boxes by the HMGB1 acidic tail results in an unstable complex with p53 until the tail is truncated to half its length, at which point the A box, proposed to be the preferred binding site for p53(1-93), is exposed, leaving the B box to bind and bend DNA. The A box interacts with residues 38-61 (TAD2) of the p53 transactivation domain. Residues 19-26 (TAD1) bind weakly, but only in the context of p53(1-93) and not as a free TAD1 peptide. We have solved the structure of the A-box/p53(1-93) complex by nuclear magnetic resonance spectroscopy. The incipient amphipathic helix in TAD2 recognizes the concave DNA-binding face of the A box and may be acting as a single-stranded DNA mimic.

PubMed: 23063560
DOI: 10.1016/j.str.2012.09.004

実験手法

SOLUTION NMR