2LXO

Identification of the Structural Traits Mediating the Antimicrobial Activity of a Chimeric Peptide of HBD2 and HBD3

2LXO の概要

• エントリーDOI: 10.2210/pdb2lxo/pdb
• NMR情報: BMRB: 18684
• 分子名称: Chimeric Peptide (1 entity in total)
• 機能のキーワード: antimicrobial peptide, designer peptide, de novo protein, antimicrobial protein
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4982.06

構造登録者

Spudy, B.,Soennichsen, F.D.,Waetzig, G.H.,Grotzinger, J.,Jung, S. (登録日: 2012-08-30, 公開日: 2013-08-14, 最終更新日: 2024-11-06)

主引用文献

Spudy, B.,Sonnichsen, F.D.,Waetzig, G.H.,Grotzinger, J.,Jung, S.
Identification of structural traits that increase the antimicrobial activity of a chimeric peptide of human beta-defensins 2 and 3.
Biochem.Biophys.Res.Commun., 427:207-211, 2012

PubMed Abstract: Antimicrobial peptides participate in the first line of defence of many organisms against pathogens. In humans, the family of β-defensins plays a pivotal role in innate immunity. Two human β-defensins, β-defensin-2 and -3 (HBD2 and HBD3), show substantial sequence identity and structural similarity. However, HBD3 kills Staphylococcus (S.) aureus with a 4- to 8-fold higher efficiency compared to HBD2, whereas their activities against Escherichia (E.) coli are very similar. The generation of six HBD2/HBD3-chimeric molecules led to the identification of distinct molecular regions which mediate their divergent killing properties. One of the chimeras (chimera C3) killed both E. coli and S. aureus with an even higher efficacy compared to the wild-type molecules. Due to the broad spectrum of its antimicrobial activity against many human multidrug-resistant pathogens, this HBD2/HBD3-chimeric peptide represents a promising candidate for a new class of antibiotics. In order to investigate the structural basis of its exceptional antimicrobial activity, the peptide's tertiary structure was determined by NMR spectroscopy, which allowed its direct comparison to the published structures of HBD2 and HBD3 and the identification of the activity-increasing molecular features.

PubMed: 22995312
DOI: 10.1016/j.bbrc.2012.09.052

実験手法

SOLUTION NMR