2LWF

Structure of N-terminal domain of a plant Grx

2LWF の概要

• エントリーDOI: 10.2210/pdb2lwf/pdb
• NMR情報: BMRB: 18624
• 分子名称: Monothiol glutaredoxin-S16, chloroplastic (1 entity in total)
• 機能のキーワード: nuclease, glutaredoxin, hydrolase
• 由来する生物種: Arabidopsis thaliana (mouse-ear cress,thale-cress)
• 細胞内の位置: Plastid, chloroplast (Potential): Q8H7F6
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12907.64

構造登録者

Feng, Y. (登録日: 2012-07-28, 公開日: 2013-05-22, 最終更新日: 2024-05-15)

主引用文献

Liu, X.,Liu, S.,Feng, Y.,Liu, J.Z.,Chen, Y.,Pham, K.,Deng, H.,Hirschi, K.D.,Wang, X.,Cheng, N.
Structural insights into the N-terminal GIY-YIG endonuclease activity of Arabidopsis glutaredoxin AtGRXS16 in chloroplasts.
Proc.Natl.Acad.Sci.USA, 110:9565-9570, 2013

PubMed Abstract: Glutaredoxins (Grxs) have been identified across taxa as important mediators in various physiological functions. A chloroplastic monothiol glutaredoxin, AtGRXS16 from Arabidopsis thaliana, comprises two distinct functional domains, an N-terminal domain (NTD) with GlyIleTyr-TyrIleGly (GIY-YIG) endonuclease motif and a C-terminal Grx module, to coordinate redox regulation and DNA cleavage in chloroplasts. Structural determination of AtGRXS16-NTD showed that it possesses a GIY-YIG endonuclease fold, but the critical residues for the nuclease activity are different from typical GIY-YIG endonucleases. AtGRXS16-NTD was able to cleave λDNA and chloroplast genomic DNA, and the nuclease activity was significantly reduced in AtGRXS16. Functional analysis indicated that AtGRXS16-NTD could inhibit the ability of AtGRXS16 to suppress the sensitivity of yeast grx5 cells to oxidative stress; however, the C-terminal Grx domain itself and AtGRXS16 with a Cys123Ser mutation were active in these cells and able to functionally complement a Grx5 deficiency in yeast. Furthermore, the two functional domains were shown to be negatively regulated through the formation of an intramolecular disulfide bond. These findings unravel a manner of regulation for Grxs and provide insights into the mechanistic link between redox regulation and DNA metabolism in chloroplasts.

PubMed: 23690600
DOI: 10.1073/pnas.1306899110

実験手法

SOLUTION NMR