2LVS

NMR solution structure of a CRISPR repeat binding protein

2LVS の概要

• エントリーDOI: 10.2210/pdb2lvs/pdb
• NMR情報: BMRB: 18589
• 分子名称: Putative uncharacterized protein (1 entity in total)
• 機能のキーワード: dna binding protein
• 由来する生物種: Hyperthermus butylicus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12239.30

構造登録者

Kenchappa, C.S.,Heidarsson, P.O.,Garrett, R.A.,Poulsen, F.M. (登録日: 2012-07-11, 公開日: 2013-01-23, 最終更新日: 2024-05-15)

主引用文献

Kenchappa, C.S.,Heidarsson, P.O.,Kragelund, B.B.,Garrett, R.A.,Poulsen, F.M.
Solution properties of the archaeal CRISPR DNA repeat-binding homeodomain protein Cbp2.
Nucleic Acids Res., 41:3424-3435, 2013

PubMed Abstract: Clustered regularly interspaced short palindromic repeats (CRISPR) form the basis of diverse adaptive immune systems directed primarily against invading genetic elements of archaea and bacteria. Cbp1 of the crenarchaeal thermoacidophilic order Sulfolobales, carrying three imperfect repeats, binds specifically to CRISPR DNA repeats and has been implicated in facilitating production of long transcripts from CRISPR loci. Here, a second related class of CRISPR DNA repeat-binding protein, denoted Cbp2, is characterized that contains two imperfect repeats and is found amongst members of the crenarchaeal thermoneutrophilic order Desulfurococcales. DNA repeat-binding properties of the Hyperthermus butylicus protein Cbp2Hb were characterized and its three-dimensional structure was determined by NMR spectroscopy. The two repeats generate helix-turn-helix structures separated by a basic linker that is implicated in facilitating high affinity DNA binding of Cbp2 by tethering the two domains. Structural studies on mutant proteins provide support for Cys(7) and Cys(28) enhancing high thermal stability of Cbp2Hb through disulphide bridge formation. Consistent with their proposed CRISPR transcriptional regulatory role, Cbp2Hb and, by inference, other Cbp1 and Cbp2 proteins are closely related in structure to homeodomain proteins with linked helix-turn-helix (HTH) domains, in particular the paired domain Pax and Myb family proteins that are involved in eukaryal transcriptional regulation.

PubMed: 23325851
DOI: 10.1093/nar/gks1465

実験手法

SOLUTION NMR