2LV7

Solution structure of Ca2+-bound CaBP7 N-terminal doman

Summary for 2LV7

• Entry DOI: 10.2210/pdb2lv7/pdb
• NMR Information: BMRB: 18557
• Descriptor: Calcium-binding protein 7, CALCIUM ION (2 entities in total)
• Functional Keywords: calcium-binding protein, metal binding protein
• Biological source: Homo sapiens (human)
• Cellular location: Golgi apparatus, trans-Golgi network membrane; Single-pass type IV membrane protein (Probable): Q86V35
• Total number of polymer chains: 1
• Total formula weight: 11481.02

Authors

Mccue, H.V.,Patel, P.,Herbert, A.P.,Lian, L.,Burgoyne, R.D.,Haynes, L.P. (deposition date: 2012-06-29, release date: 2012-09-26, Last modification date: 2024-05-15)

Primary citation

McCue, H.V.,Patel, P.,Herbert, A.P.,Lian, L.Y.,Burgoyne, R.D.,Haynes, L.P.
Solution NMR Structure of the Ca2+-bound N-terminal Domain of CaBP7: A REGULATOR OF GOLGI TRAFFICKING.
J.Biol.Chem., 287:38231-38243, 2012

PubMed Abstract: Calcium-binding protein 7 (CaBP7) is a member of the calmodulin (CaM) superfamily that harbors two high affinity EF-hand motifs and a C-terminal transmembrane domain. CaBP7 has been previously shown to interact with and modulate phosphatidylinositol 4-kinase III-β (PI4KIIIβ) activity in in vitro assays and affects vesicle transport in neurons when overexpressed. Here we show that the N-terminal domain (NTD) of CaBP7 is sufficient to mediate the interaction of CaBP7 with PI4KIIIβ. CaBP7 NTD encompasses the two high affinity Ca(2+) binding sites, and structural characterization through multiangle light scattering, circular dichroism, and NMR reveals unique properties for this domain. CaBP7 NTD binds specifically to Ca(2+) but not Mg(2+) and undergoes significant conformational changes in both secondary and tertiary structure upon Ca(2+) binding. The Ca(2+)-bound form of CaBP7 NTD is monomeric and exhibits an open conformation similar to that of CaM. Ca(2+)-bound CaBP7 NTD has a solvent-exposed hydrophobic surface that is more expansive than observed in CaM or CaBP1. Within this hydrophobic pocket, there is a significant reduction in the number of methionine residues that are conserved in CaM and CaBP1 and shown to be important for target recognition. In CaBP7 NTD, these residues are replaced with isoleucine and leucine residues with branched side chains that are intrinsically more rigid than the flexible methionine side chain. We propose that these differences in surface hydrophobicity, charge, and methionine content may be important in determining highly specific interactions of CaBP7 with target proteins, such as PI4KIIIβ.

PubMed: 22989873
DOI: 10.1074/jbc.M112.402289

Experimental method

SOLUTION NMR