2LT8

Eurocin solution structure

2LT8 の概要

• エントリーDOI: 10.2210/pdb2lt8/pdb
• NMR情報: BMRB: 18463
• 分子名称: eurocin (1 entity in total)
• 機能のキーワード: a/b-fold, cysteine stabilised, antimicrobial protein
• 由来する生物種: Eurotium amstelodami
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4348.84

構造登録者

Oeemig, J.S.,Lynggaard, C.,Knudsen, D.H.,Hansen, F.T.,Noergaard, K.D.,Schneider, T.,Vad, B.S.,Neve, S.,Kristensen, H.,Sahl, H.,Otzen, D.E.,Wimmer, R. (登録日: 2012-05-15, 公開日: 2012-10-31, 最終更新日: 2024-10-30)

主引用文献

Oeemig, J.S.,Lynggaard, C.,Knudsen, D.H.,Hansen, F.T.,Norgaard, K.D.,Schneider, T.,Vad, B.S.,Sandvang, D.H.,Nielsen, L.A.,Neve, S.,Kristensen, H.H.,Sahl, H.G.,Otzen, D.E.,Wimmer, R.
Eurocin, a New Fungal Defensin: STRUCTURE, LIPID BINDING, AND ITS MODE OF ACTION.
J.Biol.Chem., 287:42361-42372, 2012

PubMed Abstract: Antimicrobial peptides are a new class of antibiotics that are promising for pharmaceutical applications because they have retained efficacy throughout evolution. One class of antimicrobial peptides are the defensins, which have been found in different species. Here we describe a new fungal defensin, eurocin. Eurocin acts against a range of Gram-positive human pathogens but not against Gram-negative bacteria. Eurocin consists of 42 amino acids, forming a cysteine-stabilized α/β-fold. The thermal denaturation data point shows the disulfide bridges being responsible for the stability of the fold. Eurocin does not form pores in cell membranes at physiologically relevant concentrations; it does, however, lead to limited leakage of a fluorophore from small unilamellar vesicles. Eurocin interacts with detergent micelles, and it inhibits the synthesis of cell walls by binding equimolarly to the cell wall precursor lipid II.

PubMed: 23093408
DOI: 10.1074/jbc.M112.382028

実験手法

SOLUTION NMR