2LT5

Zymogen-FLG of the onconase

2LT5 の概要

• エントリーDOI: 10.2210/pdb2lt5/pdb
• NMR情報: BMRB: 17973
• 分子名称: Protein P-30 (1 entity in total)
• 機能のキーワード: ribonuclease, hydrolase
• 由来する生物種: Rana pipiens (Northern leopard frog)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13243.14

構造登録者

Vilanova, M.,Callis, M.,Laurents, D.V.,Ribo, M.,Bruix, M.,Serrano, S. (登録日: 2012-05-14, 公開日: 2012-10-24, 最終更新日: 2023-06-14)

主引用文献

(1)H, (13)C and (15)N resonance assignments of the Onconase FL-G zymogen.
Biomol.Nmr Assign., 2012

PubMed Abstract: Onconase(®) FL-G zymogen is a 120 residue protein produced by circular permutation of the native Onconase(®) sequence. In this construction, the wild type N- and C-termini are linked by a 16 residue segment and new N- and C-termini are generated at wild type positions R73 and S72. This novel segment linking the native N- and C-termini is designed to obstruct Onconase's(®) active site and encloses a cleavage site for the HIV-1 protease. As a first step towards the resolution of its 3D structure and the study of its structure-function relationships, we report here the nearly complete NMR (1)H, (13)C and (15)N resonance chemical shift assignments at pH 5.2 and 35°C (BMRB deposit no 17973). The results presented here clearly show that the structure of the wild type Onconase(®) is conserved in the FL-G zymogen.

PubMed: 22392335
DOI: 10.1007/s12104-012-9367-0

実験手法

SOLUTION NMR